中文摘要：在食品安全评估中，黄曲霉毒素B1（AFB１）是一个重要指标。本研究基于四面体DNA纳米结构(TDNs)功能化三维有序大孔MoS2-AuNPs杂交(3DOM MoS2-AuNPs) 识别接口和辣根过氧化物酶（HRP）功能化磁信号放大器，研发了一种AFB1电化学传感器。在最佳条件下，该传感器对AFB1具有特异性，线性范围良好，0.1 fg/mL-0.1 μg/mL，检测限为0.01 fg/mL。此外，将该传感器用于检测米粉及面粉样品中的AFB1含量，结果良好,与高效液相色谱-串联质谱法(HPLC-MS)一致。
外文摘要：In the food safety evaluation, aflatoxin B1 (AFB1) is an important indicator. In this work, we developed an AFB1 electrochemical aptasensor based on tetrahedral DNA nanostructures (TDNs) immobilized three dimensionally ordered macroporous MoS2-AuNPs hybrid (3DOM MoS2-AuNPs) recognition interface and horseradish peroxidase (HRP) functionalized magnetic signal amplifier. To greatly enhance the recognition efficiency, sensitivity and stability of the aptasensor, the AFB1 aptamer-incorporated TDNs were ingeniously combined with the 3DOM MoS2-AuNPs film for the construction of the sensing interface. The aptamers would release from the electrode surface after they reacted with AFB1, and then the hybridization-free TDNs formed. Thus, the biocomposite of DNA helper strands (H1)/HRP functionalized AuNPs-SiO2@Fe3O4 nanospheres would combine with the hybridization-free TDNs due to the hybridization of H1 and TDNs. The more AFB1 existed in the solution, the more H1/HRP-AuNPs-SiO2@Fe3O4 could be combined onto the 3DOM MoS2-AuNPs surface. The current response coming from HRP-catalyzed reduction of H2O2 using thionine (Thi) as electrochemical probe was proportional with the AFB1 concentration. Upon optimal conditions, the aptasensor showed specificity for AFB1, achieving a good linear range of 0.1 fg/mL-0.1 μg/mL and the detection limit of 0.01 fg/mL. Furthermore, the developed aptasensor was also applied for detecting AFB1 content in rice and wheat powder samples, obtaining good results in conformity with those achieved from the high-performance liquid chromatography tandem mass spectrometry (HPLC-MS) method.
外文关键词：three dimensionally ordered macroporous；MoS2-AuNPs film；aptasensor；tetrahedral DNA nanostructures；aflatoxin B1；rice sample
作者：Peng, G; Li, XY; Cui, F; Qiu, QY; Chen, XJ; Huang, H
作者单位：Nanjing Tech Univ
期刊名称：ACS APPLIED MATERIALS & INTERFACES
期刊影响因子：7.145
出版年份：2018
出版刊次：21
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