中文摘要：真菌和细菌及其代谢物污染会影响麦粒质量及消费者健康，本研究中，真菌和细菌DNA保守区序列分析结合单端孢霉烯族毒素和黄曲霉毒素测定，揭示了不同仓位（顶部、中部和底部）和不同的贮藏时间（3、6、9和12个月）的小麦微生物和真菌毒素。本研究为有关真菌和真菌毒素管理策略的实施和设计以及预测模型提供了资料。
外文摘要：Contamination by fungal and bacterial species and their metabolites can affect grain quality and health of wheat consumers. In this study, sequence analyses of conserved DNA regions of fungi and bacteria combined with determination of trichothecenes and aflatoxins revealed the microbiome and mycotoxins of wheat from different silo positions (top, middle, and bottom) and storage times (3, 6, 9, and 12 months). The fungal community in wheat on the first day of storage (T₀) included 105 classified species (81 genera) and 41 unclassified species. Four species had over 10% of the relative abundance: Alternaria alternata (12%), Filobasidium floriforme (27%), Fusarium graminearum (12%), and Wallemia sebi (12%). Fungal diversity and relative abundance of Fusarium in wheat from top silo positions were significantly lower than at other silo positions during storage. Nivalenol and deoxynivalenol in wheat were 13⁻34% higher in all positions at 3 months compared to T₀, and mycotoxins in wheat from middle and bottom positions at 6 to 12 months were 24⁻57% higher than at T₀. The relative abundance of toxigenic Aspergillus and aflatoxins were low at T₀ and during storage. This study provides information on implementation and design of fungus and mycotoxin management strategies as well as prediction models.
外文关键词：deoxynivalenol；fungal diversity；Fusarium graminearum；metagenomics；nivalenol；wheat storage
作者：Yuan, QS；Yang, P；Wu, AB；Zuo, DY；He, WJ；Guo, MW；Huang, T；Li, HP； Liao, YC
作者单位：Huazhong Agr Univ
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2018
出版刊次：5
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