中文摘要：本研究研制了一种视觉免疫亲和试验柱(IATC)测定法，用于无特殊仪器现场检测进行谷物样品中的伏马菌素。研究显示，所研制的IATC测定法可以作为一个有用的快速、定性及半定量现场检测谷物样品中总伏马菌素含量(FB₁、FB₂和FB₃总量)的筛选工具。
外文摘要：A visual immunoaffinity test column (IATC) assay was developed to detect fumonisins in cereal samples for spot tests without the need for special instruments. The developed IATC assay had equivalent recognition capability for fumonisin B₁ (FB₁), fumonisin B₂ (FB₂), or fumonisin B₃ (FB₃), and exhibited no cross-reactivity with aflatoxin B₁, ochratoxin A, zearalenone, or the T-2 toxin. The sample pretreatment was accomplished more rapidly and with greater ease, the entire assay procedure was completed in approximately 10 min, including sample pretreatment and testing. The limits of detection (LODs) of the IATC assay to detect fumonisins in the maize, barley, oat, and millet samples were 20 g kg1. The results of the spiked maize, barley, oat, and millet and real maize samples by the IATC assay agreed well with the results obtained by the commercial fumonisin enzyme-linked immunosorbent assay (ELISA) test kit and liquid chromatography-tandem mass spectrometry (LC-MS/MS), respectively. The developed IATC assay can serve as a useful screening tool for the rapid, qualitative, and semi-quantitative detection of the total content of fumonisins (sum of FB₁, FB₂, and FB₃) in cereal samples on-site.
外文关键词：fumonisins；immunoaffinity test column；clean-up column；visual non-instrumental detection；cereal samples
作者：Sheng, W; Wu, HS; Ji, WH; Li, Z; Chu, FY; Wang, S
作者单位：Tianjin Univ Sci & Technol
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2018
出版刊次：4
点击下载：用净化结合凝胶免疫亲和柱测定法视觉非仪器现场检测谷物样品中的伏马菌素B1、B2和B3