中文摘要：本研究描述了一种新型的黄曲霉毒素AFB1和AFB2(AFBs)磁性固相萃取(MSPE)吸附剂。用适体将磁性琼脂糖微球(MAMs)功能化，用来结合AFBs，之后用HPLC量化并进行在线柱后光化学衍生荧光检测。该方法已成功用于加标玉米样品的AFBs分析。
外文摘要：A novel adsorbent is described for magnetic solid-phase extraction (MSPE) of the aflatoxins AFB(1) and AFB(2) (AFBs). Magnetic agarose microspheres (MAMs) were functionalized with an aptamer to bind the AFBs which then were quantified by HPLC and on-line post-column photochemical derivatization with fluorescence detection. Streptavidin-conjugated MAMs were synthesized first by a highly reproducible strategy. They possess strong magnetism and high surface area. The MAMs were characterized by transmission electron microscopy, scanning electron microscopy, optical microscopy, laser diffraction particle size analyzer, Fourier transform infrared spectrometry, vibrating sample magnetometry and laser scanning confocal microscopy. Then, the AFB-aptamers were immobilized on MAMs through biotin-streptavidin interaction. Finally, the MSPE is performed by suspending the aptamer-modified MAMs in the sample. They are then collected by an external magnetic field and the AFBs are eluted with methanol/buffer (20:80). Several parameters affecting the coupling, capturing and eluting efficiency were optimized. Under the optimized conditions, the method is fast, has good linearity, high selectivity, and sensitivity. The LODs are 25 pg.mL(-1) for AFB(1) and 10 pg.mL(-1) for AFB(2). The binding capacity is 350 +/- 8 ng.g(-1) for AFB(1) and 384 +/- 8 ng.g(-1) for AFB(2), and the precision of the assay is < 8%. The method was successfully applied to the analysis of AFBs in spiked maize samples.
外文关键词：Oligonucleotide；Aptamer；Aflatoxin B-1；Aflatoxin B-2；Mycotoxin；Magnetic agarose microspheres；Biotinstreptavidin system；Magnetic solid-phase extraction；HPLC-PCD-FLD；Maize
作者：Liu, HM；Lu, AX；Fu, HL；Li, BR；Yang, MH；Wang, JH；Luan, YX
作者单位：Beijing Municipal Key Lab Agr Environm Monitoring
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2018
出版刊次：7
点击下载：HPLC测定前用适体功能化的磁性琼脂糖微球亲和捕获黄曲霉毒素B1和B2