中文摘要：用免疫分析，特别是电化学免疫分析检测黄曲霉毒素B1（AFB1），快速、灵敏而有效。本研究通过将2-氨基乙硫醇固定在Au电极表面，形成自组装单分子膜，从而提高常规电化学免疫分析法检测AFB1的速度及灵敏度。研究表明，引起电流显著变化的AFB1浓度为0.01 ng/mL。所制备的免疫传感器存放7天或再生后仍非常稳定而灵敏。
 
外文摘要：The detection of aflatoxin B1 (AFB1) using immunoassays, especially electrochemical immunoassays, is fast, sensitive and efficient. In this study, 2-aminoethanethiol was used to enhance the speed and sensitivity of conventional electrochemical immunoassays for AFB1 detection by assembly on the surface of a Au electrode, forming self-assembled monolayers (SAMs). Then, non-competitive immunoassays occurred on the modified electrode surface forming an electrochemical immunoassay sensor. X-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy were used to examine the state of the SAMs, and an electrochemical workstation was used to monitor the current change of the electrochemical reaction, so as to characterize the designed immunosensor. Our experimental results shows that, the 2-aminoethanethiol reagent was successfully assembled on the Au surface through Au-S bonding and the –NH2 terminal group faced outward. Herein, the minimum concentration of AFB1 which caused a significant current change was 0.01 ng mL−1. The prepared immunosensor also exhibited excellent stability and sensitivity after storage for 7 days or upon regeneration.
外文关键词：aflatoxin B-1；electrochemical immunoassay；2-aminoethanethiol
作者：Kong, Z；Wang, HB；Zou, L；Ji, ZG
作者单位：Hangzhou Dianzi Univ
期刊名称：:MATERIALS RESEARCH EXPRESS
期刊影响因子：0.968
出版年份：2018
出版刊次：6
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