中文摘要：玉米赤霉烯酮（ZEN）是粮食在储藏中由真菌产生的一种非甾体雌激素霉菌毒素。ZEN的早期检测设备昂贵、样品制备耗时且抗体对热敏感。本研究基于以胺官能化磁性纳米粒子（MNPS）为捕获探针标记的ZEN适体，以及以互补DNA(cDNA)为信号探针的时间分辨荧光(TRFL)纳米粒子，提出了一种新的检测策略。根据研究结果，建议采用该快速、简便的生物测定法测定食品和农产品中的ZEN。
外文摘要：Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by fungi on stored grains. The earlier detection methods used for ZEN rely on expensive equipment, time-consuming sample preparation and temperature sensitive antibodies. The current work, proposed a novel strategy based on ZEN aptamer labeled with amine-functionalized magnetic nanoparticle (MNPs) as a capture probe and time-resolved fluorescence (TRFL) nanoparticles labeled with complementary DNA (cDNA) as a signal probe. Under the optimized conditions, TRFL intensity at 544 nm was used to measure ZEN (R2 = 0.9920) in the range of 0.001–10 ng mL−1 and limits of detection (LOD) for proposed method was 0.21 pg mL−1. The specificity of bioassay was also determined by using other mycotoxins (OTA, AFB2, DON and Patulin) and results showed that the aptamer are specific to recognize only ZEN. The analytical applications of the present bioassay in maize and wheat samples were also examined and results were compared with existing methods. Based on these findings, it is suggested to use current rapid and simple bioassay for the determination of ZEN in food and agricultural products.
外文关键词：Bioassay；Zearalenone；Aptamer；Time-resolved fluorescence nanoparticles；Magnetic nanoparticles
作者：Niazi, S；Wang, XL；Pasha, I；Khan, IM；Zhao, S；Shoaib, M；Wu, SJ; Wang, ZP
作者单位：Jiangnan Univ
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2018
出版刊次：8
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