中文摘要：肉眼识别比色信号分析法有望在资源约束地区用于高通量筛选检测及现场及时诊断。本研究基于高灵敏度及稳定性的肉眼识别信号金纳米聚集，研发了一种新型直接竞争血浆酶联免疫吸附试验(dc-pELISA)，用于检测伏马菌素B1（FB1）。 结果表明，在定量检测实际玉米样品中的FB1时，该比色ELISA法能满足精度和准度的要求。
外文摘要：Naked-eye readout colorimetric signal-based assays are promising for high-throughput screening detection and point-of-care diagnostics in resource-constrained regions. Here, a novel direct competitive plasmonic enzyme-linked immunosorbent assay (dc-pELISA) based on gold nanoparticle (AuNP) aggregation with highly sensitive and robust naked-eye readout signal was developed and used to detect fumonisin B1 (FB1). AuNP aggregation was induced by a horseradish peroxidase (HRP)/hydrogen peroxide (H2O2)/tyramine (TYR) system, resulting in a dramatic color change from red to blue. In this system, H2O2 was produced via GOx-mediated glucose oxidation reaction, and FB1–GOx was used as a competitive antigen. The proposed pELISA demonstrated good linear detection of FB1 from 3.125 ng mL−1 to 25 ng mL−1 with a vivid color change from deep blue to red and a cutoff limit of 12.5 ng mL−1 observed by the naked eye. The average recoveries for FB1-spiked corn samples ranged from 76.5% to 96.8% with a relative standard derivation of 4.88%~16.4%. Meanwhile, the proposed method exhibited excellent agreement (R2 = 0.927) with the conventional ELISA method in blindly detecting FB1 spiked corn samples. Additionally, the results of the proposed method for FB1 positive corn samples also showed a high consistency with those of the ultra performance liquid chromatography method. These results indicated acceptable accuracy and precision of the proposed colorimetric ELISA for quantitative detection of FB1 in actual corn samples.
外文关键词：Plasmonic ELISA；Fumonisin B-1；Glucose oxidase；Gold nanoparticle aggregation；Tyramine
作者：Chen, XR；Liang, Y；Zhang, WJ；Leng, YK；Xiong, YH
作者单位：Nanchang Univ
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2018
出版刊次：8
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