中文摘要：本研究用活性酯法合成了脱氧雪腐镰刀菌烯醇（DON）半抗原3-O-半纤维素-脱氧雪腐镰刀菌烯醇(3-HS-DON)，并将它与牛血清白蛋白(BSA)结合，制备免疫原。用免疫原免疫的Bal b/c小鼠脾细胞融合小鼠骨髓瘤细胞系SP 2/0，制作一种新型的抗DON单克隆抗体(mAb)，对3-乙酰脱氧雪腐镰刀菌烯醇（3-ADON）和15-乙酰脱氧雪腐镰刀菌烯醇（15-ADON）显示出良好的交叉反应性。将mAb与琼脂糖凝胶4B偶联，填充到固相萃取（SPE）筒中，制备免疫亲和柱(IAC)。便建立了同时测定谷物中DON、3-ADON和15-ADON的方法。该方法简单、快速、选择性好、灵敏度高、环境友好，适合用来测定谷物中的DON、3-ADON和15-ADON。
外文摘要：Deoxynivalenol (DON) hapten 3-O-hemisuccinyl-deoxynivalenol (3-HS-DON) was synthesized and conjugated to bovine serum albumin (BSA) to prepare immunogen by active ester method. A novel anti-DON monoclonal antibody (mAb) showing good cross-reactivity against both 3-acetyl-deoxynivalenol (3-ADON) and l5-acetyl-deoxynivalenol (15-ADON) was produced by fusing mouse myeloma cells lines SP 2/0 with spleen cells from Bal b/c mice which were immunized by the immunogen. The mAb was coupled with sepharose-4B, then packed into a solid phase extraction (SPE) cartridge to prepare an immunoaffinity columns (IAC). A method for simultaneous determination of DON, 3-ADON, and 15-ADON in cereals was developed. The cereal samples were extracted by water, cleaned up with IAC, and determined by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). The results showed that the limits of detection (LODs) for DON, 3-ADON, and 15-ADON were 1, 6, and 3 μg kg−1, respectively. Recoveries of DON, 3-ADON, and 15-ADON from wheat, oatmeal, and maize were 67.5–93.8, 63.8–113.2, and 75.5–106.6% at spiking levels of 20–2000 μg kg−1, and the relative standard deviations (RSDs) were 2.4–13.7, 2.4–12.6, and 7.6–12.2%, respectively. This method was suitable for determination of DON, 3-ADON, and 15-ADON in cereals, with the advantages of simplicity, rapidness, good selectivity and sensitivity, and environmental friendliness.
外文关键词：Monoclonal antibody；Immunoaffinity column；Deoxynivalenol；3-acetyl-deoxynivalenol；15-acetyl-deoxynivalenol；HPLC-MS/MS
作者：Zuo, HG；Zhu, JX；Shi, L；Zhan, CR；Guo, P；Wang, Y；Zhang, YM；Liu, JP
作者单位：Jiangxi Entry Exit Inspect & Quarantine Bur
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2018
出版刊次：8
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