中文摘要：本研究开发了一种新型磁性固相萃取(MSPE)结合高效液相色谱（HPLC）的方法，用来同时检测黄曲霉毒素B1(AFB1)和玉米赤霉烯酮(ZON)。这种新型磁萃取绿色MSPE-HPLC法对分析复杂基质样品中的霉菌毒素潜力巨大。
外文摘要：A novel magnetic solid-phase extraction (MSPE) coupled with high performance liquid chromatography (HPLC) method was explored for aflatoxin B1 (AFB1) and zearalenone (ZON) simultaneous determination. This method was based on a newly explored extractant of Fe3O4 nanoparticle-filled amino-modified multi-walled carbon nanotubes (Fe3O4-MWCNTs-NH2) composite, which was sensitive to analytes with structure of multi-aromatic rings and abundant hydrogen bond acceptors, based on the extraction mechanism of π–π EDA and hydrogen-bonding interactions. The morphology, structure and magnetic properties of the extractant were characterized by transmission electron microscopy (TEM), Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD) and vibrating sample magnetometry (VSM). Adsorption isotherms and kinetics for two mycotoxins were investigated and proved to be the best fit by Dubinin–Astakhov and pseudo-second-order models. Critical MSPE parameters, including solution pH, extraction time, salt addition and temperature, were investigated and optimized and the proposed analytical method was validated. Subsequently, the method was successfully applied for AFB1 and ZON analysis from wheat flour samples. The proposed method proved to be an excellent green analysis with a score of 89 in analytical Eco-Scale evaluation. It also has good linearities in the range of 1.0–100.0 ng g−1 with linear coefficients (r) above 0.99, desirable recoveries ranging from 88.8% to 96.0%, satisfactory precision with relative standard deviation (RSD) in the range of 2.1–2.8%, and low limits of detection (LODs) of 0.15 and 0.24 ng g−1 for AFB1 and ZON, respectively. This novel magnetic-extractant-based green MSPE-HPLC method has great potential for mycotoxin analysis from complex matrix samples.
作者：Li, WK；Zhang, HX；Shi, YP
作者单位：Chinese Acad Sci
期刊名称：ANALYTICAL METHODS
期刊影响因子：1.915
出版年份：2018
出版刊次：27
点击下载：用磁性纳米粒子填充的氨基改性多壁碳纳米管同时测定黄曲霉毒素B1和玉米赤霉烯酮