中文摘要：酶联免疫吸附法(ELISA)被用作监测食品和动物饲料中致癌物伏马菌素B1(FB1)的标准工具。但由于免疫分析中抗体及酶的稳定性、样品中存在的天然酶抑制剂，以及高水平的非特异性蛋白结合等问题，ELISA并非总能奏效。此外，由于要用到动物，ELISA抗体的制备耗时耗财。为了克服这些限制因素，本研究开发了一种分子印迹纳米粒分析法(MINA)，用分子印迹纳米粒(nanoMIPs)代替了竞争性ELISA中的第一抗体。研究表明，NINA 检测FB1的浓度范围为10 pM–10 nM，检测限为1.9 pM，回收率为108.13–113.76%。
外文摘要：The enzyme-linked immunosorbent assay (ELISA) has been used as a standard tool for monitoring food and animal feed contamination from the carcinogenic fumonisin B1 (FB1). Unfortunately, ELISA is not always efficient due to the instability of the antibody and enzyme components in the immunoassay, the presence of natural enzyme inhibitors in the samples and the high levels of non-specific protein binding. Additionally, the production of antibodies for ELISA can be time-consuming and costly, due to the involvement of animals in the manufacturing process. To overcome these limiting factors, a molecularly imprinted nanoparticle based assay (MINA) has been developed, where the molecularly imprinted nanoparticles (nanoMIPs) replace the primary antibody used in a competitive ELISA. Herein, computational modelling was used to design the nanoMIPs by selecting monomers that specifically interact with FB1. The affinity of the monomers to FB1 was verified by measuring their binding in affinity chromatography experiments. The nanoMIPs were produced by solid phase synthesis and the results showed that nanoMIPs had a hydrodynamic diameter of around 249 ± 29 nm. The assay tested in model samples is highly selective and does not show cross-reactivity with other mycotoxins such as fumonisin B2 (FB2), aflatoxin B1 (AFB1), citrinin (CTT), zearalenone (ZEA), and deoxynivalenol (DON). The MINA allows the detection of FB1 in the concentration range of 10 pM–10 nM with a detection limit of 1.9 pM and a recovery of 108.13–113.76%.
作者：Munawar, H；Smolinska-Kempisty, K；Cruz, AG；Canfarotta, F；Piletska, E；Karim, K；Piletsky, SA
作者单位：Univ Leicester
期刊名称：ANALYST
期刊影响因子：4.033
出版年份：2018
出版刊次：14
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