中文摘要：用益生菌作为防止黄曲霉毒素B1（AFB1）这种食物污染物的膳食措施已大大地增加。一些研究发现AFB１结合细菌细胞壁具有菌株特异性。此外，AFB1与细菌细胞壁之间的相互作用尚不明了，因此需要做进一步研究。本研究旨在评估不同浓度的代田菌(Lcs)结合黄曲霉毒素的能力，并确定Lcs细胞的不同成分，包括活细胞、热处理及细胞壁，结合AFB1的效率。此外，还通过扫描电子显微镜（SEM）和动物研究评估了AFB1与Lcs之间的相互作用。结果表明，Lcs具有强大的结合能力，凸显了其作为黄曲霉毒素中毒预防剂的潜在用途。
外文摘要：The use of probiotic as dietary approach to prevent exposure to food contaminant, aflatoxin B1 (AFB1) has greatly increased. Several studies found that AFB1 binding to the bacterial cell wall is strain-specific. Moreover, the interaction between AFB1 and bacterial cell wall is not well-understood, thus warrants further investigation. This research was conducted to assess the ability of Lactobacillus casei Shirota (Lcs) to bind AFB1 at different concentrations and to determine AFB1 binding efficiency of different Lcs cell components including live cell, heat-treated, and cell wall. In addition, the interaction between AFB1 and Lcs was also evaluated via scanning electron microscopy (SEM) and through an animal study. The binding of AFB1 by all Lcs cell components depends on the concentration of available AFB1. Among all Lcs cell components, the live Lcs cells exhibited the highest binding efficiency (98%) toward AFB1. Besides, the SEM micrographs showed that AFB1 induced structural changes on the bacterial cell surface and morphology including rough and irregular surface along with a curve rod-shaped. In vivo experiment revealed that Lcs is capable to neutralize the toxicity of AFB1 on body weight and intestine through the binding process. The animal's growth was stunted due to AFB1 exposure, however, such effect was significantly (p < 0.05) alleviated by Lcs. This phenomenon can be explained by a significant (p < 0.05) decreased level of blood serum AFB1 by Lcs (49.6 ± 8.05 ng/mL) compared to AFB1-exposed rats without treatment (88.12 ± 10.65 ng/mL). Taken together, this study highlights the potential use of Lcs as a preventive agent against aflatoxicosis via its strong binding capability.
外文关键词：AFB1 binding efficiency；Lactobacillus casei Shirota；adsorption isotherm；scanning electron microscopy；morphology；serum AFB1
作者：Liew, WPP；Nurul-Adilah, Z；Than, LTL；Mohd-Redzwan, S
作者单位：Univ Putra Malaysia
期刊名称：FRONTIERS IN MICROBIOLOGY
期刊影响因子：4.165
出版年份：2018
出版刊次：7
点击下载：代田菌对黄曲霉毒素B1的结合效率及相互作用