中文摘要：优秀的信号放大策略，酪胺信号放大(TSA)，能提高分析物分析的灵敏度。本研究通过与TSA系统及进一步的生物素链霉亲和素（BSA）系统结合，提高了酶联免疫吸附测验(ELISA)的灵敏度，研制了一种基于TSA的灵敏TSA-ELISA法，用来检测食用油样品中的黄曲霉毒素B1（AFB1）。本研究为提高简单实用的ELISA方法的灵敏度提供策略，极具推广价值及应用前景。
外文摘要：Tyramine signal amplification (TSA), the excellent signal amplification strategy, has the potential to improve the sensitivities of analytes analysis. In this work, the sensitivity of enzyme-linked immunosorbent assays (ELISA) has significantly improved by coupling TSA system and further biotin-streptavidin (BSA) system. Thus, a sensitive TSA-ELISA based on TSA was developed for detecting aflatoxin B1 (AFB1) in edible oil samples. Under optimal conditions, the limit of detection (LOD, IC10) and the half-maximal inhibition concentration (IC50) of the TSA-ELISA were 0.004 and 0.039 ng/mL for AFB1, respectively. The developed TSA-ELISA for AFB1 has an 11-fold improved LOD value and 6-fold improved IC50 value when compared with ELISA. The cross-reactivities of the TSA-ELISA with its analogues were negligible (1 in edible oil samples. Furthermore, the results of TSA-ELISA correlated well with those obtained by HPLC-fluorescence detector. The proposed TSA-ELISA was a satisfactory tool for sensitive, inexpensive, high-throughput, and alternative detection of AFB1 in edible oil samples. This study could provide the strategy for improving the sensitivity of ELISA with simple and practical approach, which has significant popularizing value and application prospect.
外文关键词：Aflatoxin B-1；Tyramine signal amplification；Biotin-streptavidin；ELISA；Edible oil samples
作者：Zhang, YY；Li, M；Cui, Y；Hong, X；Du, DL
作者单位：Jiangsu Univ
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2018
出版刊次：9
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