中文摘要：脱氧雪腐镰刀菌烯醇（DON）是一种由镰刀菌产生的蛋白质合成抑制剂，它频繁地污染人畜食用的粮食，因而必须进行有效监测和控制。基于抗体的免疫分析为筛选该霉菌毒素提供了高效廉价的方法。为了实现高灵敏度并避免酶促反应，本研究制备了一种高度灵敏的单克隆抗体(MAb)及一种包含了氨基官能化核/多壳层量子点(QDs)，CdSe/CdS/Cd0.5Zn0.5S/ZnS与3H-Don BSA生物偶联的荧光探针；由此研制了一种灵敏而可靠的直接竞争荧光标记免疫吸附试验(dcFLISA)法。研究表明，该方法作为一种简单有效的小分子现场筛选策略具有广阔的应用前景。
外文摘要：Deoxynivalenol (DON) is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption, thereby necessitating their effective monitoring and control. Antibody-based immunoassays provide efficient and cheap methods for screening this mycotoxin. To achieve high sensitivity and avoid enzymatic reaction, a new highly sensitive monoclonal antibody (MAb) and a fluorescent probe consisting of amino-functionalized core/multishell quantum dots (QDs), CdSe/CdS/Cd0.5Zn0.5S/ZnS, bio conjugated to 3-HS-DON-BSA were prepared; then, a sensitive and reliable direct competitive fluorescent-labeled immunosorbent assay (dcFLISA) was developed. Under optimized conditions, the 50% inhibition concentration (IC50) value of the dcFLISA was 5.6 μg/kg with limit of detection of 12.2 μg/kg in maize samples. The recoveries ranged from 77.3 to 84.3% with coefficients of variation (CV%) values below 10%. Good correlation between the dcFLISA and HPLC-MS/MS results in the naturally contaminated maize demonstrated that the dcFLISA was a rapid and sensitive with high-performance and reliable method for the detection of DON. The potential of this method to function as a simple and efficient strategy for small molecule on-site screening might be applied in the future.
外文关键词：Monoclonal antibody；Deoxynivalenol；Quantum dots；Direct competitive fluorescent-labeled immunosorbent assay
作者：Zhang, XY；Yu, XZ；Wang, JY；Wang, Q；Meng, H；Wang, ZH
作者单位：Henan Agr Univ
期刊名称：FOOD ANALYTICAL METHODS
期刊影响因子：2.167
出版年份：2018
出版刊次：9
点击下载：用核/多壳层量子点荧光免疫分析法一步筛选玉米中的脱氧雪腐镰刀菌烯醇