中文摘要：本研究研制了一种可靠而快捷的方法，用来测定四种饲料，包括碎米、花生、玉米和鱼粉中的黄曲霉毒素B1(AFB1)。该方法采用QuEChERS法制备样品，省去净化步骤。用乙腈/甲醇(40/60 v/v)结合氯化钠与硫酸镁的分配来提取AFB1。用高效液相色谱法结合柱前衍生化与荧光检测来测定AFB1。结果表明该方法可用于饲料AFB1污染的定期监测。
外文摘要：A reliable and rapid method has been developed for the determination of aflatoxin B1 (AFB1) in four kinds of feedstuffs comprising broken rice, peanuts, corn, and fishmeal. A sample preparation was carried out based on the QuEChERS method with the exclusion of the clean-up step. In this study, AFB1 was extracted using acetonitrile/methanol (40/60 v/v), followed by partitioning with sodium chloride and magnesium sulfate. High-performance liquid chromatography with precolumn derivatization and fluorescence detection was performed. The coefficients of determination were greater than 0.9800. Throughout the developed method, the recovery of all feedstuffs achieved a range of 82.50-109.85% with relative standard deviation lower than 11% for all analytes at a concentration of 20-100 ng/g. The limit of detection (LOD) ranged from 0.2 to 1.2 ng/g and limit of quantitation (LOQ) ranged from 0.3 to 1.5 ng/g. The validated method was successfully applied to a total of 120 samples. The occurrence of AFB1 contamination was found at the following concentrations: in broken rice (0.44-2.33ng/g), peanut (3.97-106.26ng/g), corn (0.88-50.29 ng/g), and fishmeal (1.06-10.35 ng/g). These results indicate that the proposed method may be useful for regularly monitoring AFB1 contamination in feedstuffs.
作者：Choochuay, S；Phakam, J；Jala, P；Maneeboon, T；Tansakul, N
作者单位：Kasetsart Univ
期刊名称：INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY
期刊影响因子：0.719
出版年份：2018
出版刊次：-
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