中文摘要：本研究通过免疫和细胞克隆，获得了三个杂交瘤细胞系，能分泌抗伏马菌素B1（FB1）的单克隆抗体，特别是抗体亚型IgA（重链）和k（轻链）。用硫基烷酸(MUA)对金纳米粒子(AuNPs)表面进行改性得到AuNPs-MUA，用它作辣根过氧化物酶（HRP）-山羊抗小鼠IgA的载体，研制一种增强间接竞争酶联免疫吸附试验(IC-ELISA)，检测FB1。研究表明，AuNP增强的IC-ELISA具有很好的精度和稳定性，使其成为一种定量检测玉米中伏马菌素（FB1、FB2和FB3）总含量的方案。
外文摘要：In this paper, three hybridoma cell lines that secrete monoclonal antibodies against fumonisin B-1 (FB1), specifically antibody subtypes IgA (heavy-chain) and kappa (light-chain), were obtained by immunization and cell cloning procedures. And the surfaces of gold nanoparticles (AuNPs) were modified with mercaptoundecanoic acid (MUA) to obtain AuNPs-MUA, which was used as a carrier for horseradish peroxidase (HRP)-goat anti-mouse IgA to develop an enhanced indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) for the detection of FB1. The 50% inhibitory concentration (IC50) and the limit of detection (LOD, 15% inhibitory concentration, IC15) of this method were 0.93 +/- 0.058 and 0.078 +/- 0.013 g L-1, respectively. The detection sensitivity was approximately 10 times higher than that of the traditional IC-ELISA (IC50 is 9.57 g L-1). The cross-reactivity rates of AuNP enhanced IC-ELISA with FB1, FB2, and FB3 were 100%, 86.9%, and 79.5%, respectively, and did not cross with other mycotoxins. The total amount of fumonisins (FB1, FB2, and FB3) in actual contaminated maize samples was determined by AuNP enhanced IC-ELISA, and the results were consistent with liquid chromatography-tandem mass spectrometry (LC-MS/MS) with R-2 = 0.9989. AuNP enhanced IC-ELISA showed good accuracy and stability, which made it one of the solutions to quantitatively detect the total content of fumonisins (FB1, FB2, and FB3) in maize.
作者：Li, Z; Sheng, W；Liu, Q；Li, SJ；Shi, YJ；Zhang, Y；Wang, S
作者单位：Nankai Univ
期刊名称：ANALYTICAL METHODS
期刊影响因子：1.915
出版年份：2018
出版刊次：28
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