中文摘要：黄曲霉毒素是一大类毒性、诱变性及致癌性都很强的霉菌毒素，由真菌某些特殊种类产生。这些化合物对食品的潜在污染造成广泛的损害，从而造成巨大的经济损失。本研究探索了从黄曲霉和寄生曲霉中筛选的短乳杆菌和副乳杆菌产生的抗菌化合物，对抑制产毒真菌生长及随后的黄曲霉毒素B1产生的潜在应用。结果证实了乳酸菌是控制发酵食品和乳制品污染的一种很有前景的策略。
外文摘要：Aflatoxins are a large group of highly toxic, mutagenic, and carcinogenic mycotoxins produced by specific species of fungi. Potential contamination of food commodities by these compounds causes extensive damage that lead to great economic losses. This study explored the potential use of antifungal compounds, produced by Lactobacillus brevis and Lactobacillus paracasei, for growth inhibition and subsequent aflatoxin B1 production from select strains of Aspergillus flavus and Aspergillus parasiticus. Lactobacilli strains were isolated from traditional Egyptian dairy products, whereas fungal strains were isolated from infected cereal seeds. There were noticeable decreases in mycelium biomass and aflatoxin production as well. L. brevis exhibited the highest reduction of aflatoxin B1 production by A. flavus and A. parasiticus, 96.31 and 90.43%, respectively. The concentrations of amino acids of the antifungal compound produced by L. brevis were significantly higher than that produced by L. paracasei. Asparagine, glutamine, glycine, alanine, and leucine were the most concentrated amino acids for both strains. The antifungal compounds produced by L. brevis and L. paracasei were active in a wide range of pH, heat stable and inactivated by proteolytic enzymes (protease K and trypsin A). The expression of Omt-A gene that involved in the later step of aflatoxin production was evaluated by real-time PCR. There was a vigorous reduction at transcriptional level of Omt-A gene observed in A. flavus that is treated by L. brevis and L. paracasei (80 and 70%, respectively). However, the reduction of Omt-A gene observed in A. parasiticus that is treated by L. brevis and L. paracasei was 64.5 and 52%, respectively. Treating maize seeds with antifungal compounds exhibited great efficiency in controlling fungal infection and increasing seed germination. The results confirmed that lactic acid bacteria are a promising strategy to control food contamination of fermented food and dairy products.
外文关键词：Lactobacillus sp.；Aflatoxin B1；A. flavus；A. parasiticus；Omt-A gene
作者：Gomaa, EZ；Abdelall, MF；El-Mahdy, OM
作者单位：Ain Shams Univ
期刊名称：PROBIOTICS AND ANTIMICROBIAL PROTEINS
期刊影响因子：1.283
出版年份：2018
出版刊次：2
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