中文摘要：作为毒性最强的霉菌毒素之一，黄曲霉毒素B1（AFB1）是主要的食品污染物，对人体健康构成高风险。本研究基于G-四链寡核苷酸适体嵌合体发夹结构、链霉亲和素包覆二氧化硅纳米颗粒(SNPs-Streptavidin)及N-甲基中卟啉IX(NMM)，提出了一种检测AFB1的精确荧光传感法。本研究构建的适体传感器成功地用于评估加标葡萄汁和人血清样品中的AFB1。葡萄汁样品中AFB1的分析回收率为95-106%，表明该适体传感器在食品分析中潜力巨大。
外文摘要：As one of the most toxic mycotoxins, aflatoxin B1 (AFB1) is a major food pollutant which can pose a high risk to human health. In this work, an accurate fluorescent sensing method was proposed for AFB1 determination, based on hairpin structure of G-quadruplex oligonucleotide-Aptamer chimera, silica nanoparticles coated with streptavidin (SNPs-Streptavidin) and N-methyl mesoporphyrin IX (NMM). The hairpin structure of chimera and SNPs-Streptavidin allowed AFB1 detection with high sensitivity and specificity. Moreover, the developed sensor could detect AFB1 in 30 min. The relative fluorescence intensity increased as AFB1 concentrations increased with a linear range of 30-900 pg/mL and a limit of detection (LOD) of 8 pg/mL. The constructed aptasensor was successfully employed to assess AFB1 spiked grape juice and human serum samples. The analytical recovery of AFB1 in the grape juice samples ranged from 95-106%, implying the great potential of the presented aptasensor in food product analysis.
外文关键词：Fluorescent aptasensor；Chimera；Aflatoxin B-1；N-Methyl mesoporphyrin IX；Silica nanoparticles
作者：Taghdisi, SM；Danesh, NM；Ramezani, M；Abnous, K
作者单位：Mashhad Univ Med Sci
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2018
出版刊次：12
点击下载：用一种新型的基于G-四链寡核苷酸适体嵌合体发夹结构和二氧化硅纳米粒子的放大荧光传感器灵敏检测葡萄汁中的黄曲霉毒素B1