中文摘要：本研究描述了一种通过酶免疫夹心法检测苋菜籽中玉米赤霉烯酮（ZEA）的电化学免疫传感器。研究表明，用该方法检测苋菜籽玉米赤霉烯酮，获得了良好的选择性、稳定性及再现性。
外文摘要：We describe an electrochemical immunosensor for zearalenone (ZEA) determination in Amaranthus cruentus seeds by an enzyme immunoassay sandwich type. The device is based on a screen-printed carbon electrode (SPCE) modified with amino mesoporous silica (MCM-41) synthetized with Fe2O3in situ. Mesoporous material enlarges the surface available for anti-ZEA antibodies immobilization. SPCE/MCM-41-Fe2O3 was characterized by scanning electron microscopy, energy dispersive X-ray spectroscopy, and cyclic voltammetry. ZEA in the sample previously pretreated was recognized and captured by anti-ZEA on SPCE/MCM-41-Fe2O3. Then, the horseradish peroxidase (HRP)-conjugated anti-ZEA-antibody was added, and the substrate solution (H2O2 + 4-terbutylcatechol (4-TBC)) reacted with the HRP that catalyzed the oxidation of 4-TBC to 4-terbutylbenzoquinone (TBQ). Finally, the enzymatic product was detected at −100 mV, and the current was proportional to the ZEA present in the sample. The calibration plot exhibited a linear range from 1.88 to 45 ng mL−1, and the limit of detection was 0.57 ng mL−1 (r2 = 0.998). The coefficient of variation inter- and intra assay was below 6%. Our method achieved a good selectivity, stability and reproducibility for ZEA detection in A. cruentus seeds.
外文关键词：Electrochemical；Immunosensor；Mesoporous silica；Zearalenone；Amaranthus cruentus seeds
作者：Regiart, M；Fernandez, O；Vicario, A；Villarroel-Rocha, J；Sapag, K；Messina, GA；Raba, J；Bertolino, FA
作者单位：Univ Nacl San Luis
期刊名称：MICROCHEMICAL JOURNAL
期刊影响因子：2.893
出版年份：2018
出版刊次：9
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