中文摘要：适体，因其对目标分析物固有的高选择性，有望成为纳米粒子表面改性的候选材料。本研究制备了适体功能化的磁性纳米粒子(AMNPs)，并用其改进了磁性固相萃取法，在用高效液相色谱法测定黄曲霉毒素M1(AFM1)含量前，用来净化牛奶和乳制品样品。研究表明，该新型优良吸附剂在简化样品的复杂基质方面潜力巨大，并可用来检测、富集和准确测定超痕量的AFM1。
外文摘要：Aptamers, due to the inherently high selectivity towards target analytes, are promising candidate for surface modification of the nanoparticles. Therefore, aptamer-functionalized magnetic nanoparticles (AMNPs) was prepared and used to develop a magnetic solid-phase extraction procedure for clean-up of milk and dairy products samples before measuring the aflatoxin M1 (AFM1) contents by the high-performance liquid chromatography. The prepared sorbent was characterized by different instrumental methods such as FT-IR, FESEM, TEM, EDX and AGFM. The AMNPs was used in extraction and pre-concentration of ultratrace amounts AFM1 from local milk samples. The amount of sorbent, elution volume, extraction time, and salt addition were optimized. Based on the results, calibration plot is linear over the 0.3 to 1 ng·L−1 and 5 to 50 ng·L−1 AFM1 concentration ranges. The limits of detection of the developed method were obtained 0.2 ng·L−1 which is the smallest value that has been reported up to now. The results show that this new superior sorbent has a large potential to simplify the complex matrix of the samples and can used for detection, preconcentration and accurate determination of ultratrace amounts of the AFM1 from milk and dairy products.
外文关键词：Aptamer；Aflatoxin M1；Magnetic nanoparticles；HPLC；Solid phase extraction
作者：Khodadadi, M；Malekpour, A；Mehrgardi, MA
作者单位：Univ Isfahan
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2018
出版刊次：8
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