中文摘要：有人发现发射峰为459nm的碳点（CD）荧光被吸收峰为430nm的纳米银粒子（AgNPs）强烈猝灭。以CDs结合卵清蛋白(OVA)作供体探针，AgNP-Ab作受体探针，将该发现用于荧光猝灭侧流免疫层析法，检测玉米赤霉烯酮(ZEN)。研究表明，该方法代表了一种简单、灵敏、快速测定谷物样品及其制品中ZEN的工具。
外文摘要：It is found that the fluorescence of carbon dots (CD) with an emission peak at 459 nm is strongly quenched by silver nanoparticles (AgNPs) with their absorption peak at 430 nm. The finding was applied in a fluorescence quenchometric lateral flow immunochromatographic assay for detection of zearalenone (ZEN) with CDs conjugated to ovalbumin (OVA) as donor signal probe and AgNP-Ab as acceptor signal probe. The assay has an LOD of 0.1 μg·L-1 for ZEN. This is 10 times better than the respective "turn-off" AgNP-based LFIA. In case of cereal samples and their products, the LODs range from 1 to 2.5 μg·kg-1. Only minor cross reactivity is found for fusarium toxins, and no cross-sensitivity for aflatoxin B1, T-2 mycotoxin, ochratoxin A, deoxynivalenol, and fumonisin B1. The assay represents a simple, sensitive, and rapid tool for determination of ZEN in cereal samples and their products.
外文关键词：Carbondot；Silvernanoparticle；Fluorescence quenching；"Turnon"；Immunochromatographic strip；Zearalenone；Cereal samples and their products
作者：Li, SJ；Wang, JP；Sheng, W；Wen, WJ；Gu, Y；Wang, S
作者单位：Tianjin Univ Sci & Technol
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2018
出版刊次：8
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