中文摘要：本研究采用多元曲线分辨-交替最小二乘 (MCR-ALS) 算法，初步分析了黄曲霉毒素B1(AFB1)不同程度污染-无污染、低污染及高污染以及当地销售的玉米粉样品的激发发射光谱（EEM）。研究表明荧光EEM结合MCR-ALS可用来快速简便地评估玉米粉的AFB1污染程度。
外文摘要：In this preliminary study, we used the Multivariate Curve Resolution- Alternating Least Squares (MCR-ALS) algorithm to analyze the excitation-emission matrix for different samples of maize flour contaminated with aflatoxin B1 (AFB1) - uncontaminated, low-contaminated, high-contaminated and flour from the local market. We intended to see if there are differences in emission spectral parameters that depend on degree of contamination. The analysis used genuine emission of the fluorophores in the flour, in absence and presence of AFB1, which enables fast screening of the samples, without sample pre-processing. As a result of the analysis, two fluorescence components were derived from the emission spectra for all analyzed samples. The components’ positions were the same for the uncontaminated reference sample and the commercial flour sample from the local market, whereas for the samples contaminated with the aflatoxin B1, the emitted peaks’ positions were red-shifted. We found that the ratio of the areas of these two components is proportional to the intensity of contamination: 0.071 for uncontaminated sample, 0.090 for the sample from local market, 0.192 for low-contaminated sample and 1.431 for high-contaminated sample. These results indicate that fluorescence EEM coupled with MCR-ALS could be used for rapid and simple estimation of the degree AFB1 contamination in maize flour.
外文关键词：Steady state fluorescence spectroscopy, Excitation-emission matrix, MCR-ALS, Aflatoxin B1；Maize flour
作者：Bartolic, D；Stankovic, M；Mutavdzic, D；Stankovic, S；Jovanovic, D；Radotic, K
作者单位：Univ Belgrade
期刊名称：JOURNAL OF FLUORESCENCE
期刊影响因子：1.601
出版年份：2018
出版刊次：3
点击下载：用多元曲线分辨-激发发射光谱的交替最小二乘分析检测黄曲霉毒素B1污染的玉米粉