中文摘要：黄曲霉毒素B1(AFB1)和玉米赤霉烯酮(ZEA)是广泛污染食物和饲料的真菌产生的有毒次生代谢物。限制人类和牲畜对它们的暴露非常必要。在大量的净化霉菌毒素的方法中，微生物和酶法生物降解是消除危害的有效途径。本研采究用响应面法(RSM)，旨在优化不同种类有益微生物的比例，并将其与霉菌毒素降解酶进行结合。结果表明，益生菌结合霉菌毒素降解酶是一种很有前途的同步降解AFB1和ZEA的新方法。
外文摘要：Aflatoxin B-1 (AFB(1)) and zearalenone (ZEA) are the secondary toxic metabolites of fungi which contaminate a wide range of food and feedstuffs. Limiting exposure of humans and livestock to them is very essential. Among numerous methods of mycotoxin-degradation, biodegradation by microorganisms and enzymes is an effective and promising approach to eliminate their hazards. The present study aims to optimize the proportion of different species of beneficial microbes by means of response surface methodology (RSM) and its combination with mycotoxin-degradation enzymes. The results indicated that AFB(1) and ZEA degradation rates were 38.38% and 42.18% by individual Bacillus subtilis (P < 0.05); however, AFB(1) and ZEA degradation rates reached 45.49% and 44.90% (P < 0.05) when three probiotic species such as Bacillus subtilis, Lactobacillus casein and Candida utilis were at a ratio of 1:1:1, corresponding with the predictive value of the RSM model. The further experiment showed that AFB(1) and ZEA degradation rates were 63.95% and 73.51% (P < 0.05) when the compound of three probiotic species was combined with mycotoxin-degradation enzymes from Aspergillus oryzae at a ratio of 3:2. This result indicated that the combination of probiotics with mycotoxin-degradation enzymes is a promising new approach for synchronous detoxification of AFB(1) and ZEA.
外文关键词：Biodegradation；Mycotoxin；probiotics；mycotoxin-degradation enzyme；Response surface methodology
作者：Huang, WW；Chang, J；Wang, P；Liu, CQ；Yin, QQ；Zhu, Q；Lu, FS；Gao, TZ
作者单位：Henan Agr Univ
期刊名称：JOURNAL OF TOXICOLOGICAL SCIENCES
期刊影响因子：1.224
出版年份：2018
出版刊次：4-6
点击下载：复合益生菌结合霉菌毒素降解酶对净化黄曲霉毒素B1及玉米赤霉烯酮的作用