中文摘要：由于霉菌毒素污染储藏粮食是一个重要问题，本研究旨在评估谷仓环境中黄曲霉毒素B1(AFB1)的预测性监测。运用储藏测试，研究了杂色曲霉素(Ver A, AFB1生物合成中的一个中间体)水平与产黄曲霉毒素真菌之间以及它们与黄曲霉毒素产生之间的相关性。结果表明，即使当AFB1水平非常低时，监测Ver A水平也可能增加粮食消费的安全性，特别是考虑到Ver A的毒性。
外文摘要：The objective of this study was to evaluate the feasibility of the predictive monitoring of aflatoxin B1 (AFB1) under granary conditions, since mycotoxin contamination of the stored grain represents an important issue. Using the storage test, we investigated the relationship between versicolorin A (Ver A, an intermediate in AFB1 biosynthesis) levels and the levels of aflatoxigenic fungi, and their relationship with aflatoxin production. All samples, except for one, were found to be contaminated with aflatoxigenic fungi using PCR analyses, while their AFB1 levels were not detectable before the storage test using an ELISA method with an LOD of 2 μg/kg. Aflatoxigenic fungi levels were analysed, as well as Ver A levels prior to the accumulation of AFB1. Levels were ≥5 μg/kg; the permissible levels of AFB1 in corn intended for direct consumption are 1˂5μg/kg) times are significantly correlated with the Ver A levels and the changes in Ver A levels (ΔVer A). Both high and variable Ver A levels were indicative of the vigorous metabolic activity of aflatoxigenic fungi. In contrast, steady Ver A levels showed that aflatoxin production by the fungi was not active. Monitoring Ver A levels and their changes may allow an earlier detection of harmful aflatoxin contamination in the stored grain. Additionally, the toxicity of Ver A should be further examined. The results of our study indicate that the monitoring of Ver A levels, even when the AFB1 levels are very low, may increase the safety of grain consumption, especially considering Ver A toxicity.
外文关键词：Corn storage；versicolorin A；aflatoxin B-1；early risk evaluation；indicator
作者：Zhang, SY；Wang, H；Yang, M；Yao, DS；Xie, CF；Liu, DL
作者单位：Jinan Univ
期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
期刊影响因子：1.878
出版年份：2018
出版刊次：5
点击下载：杂色曲霉素A是粮仓储藏玉米黄曲霉毒素污染的潜在标志物