中文摘要：本研究研制了基于适体功能化纳米金粒子着色剂的侧向横流免疫层析试纸条，用来检测微量污染物黄曲霉毒素B1（AFB1）和氯霉素（CAP）。研究表明该侧向横流免疫层析试纸条可以作为简单、快捷而有效的筛选工具，用来现场快速检测各种微量污染物。
外文摘要：Lateral flow immunochromatographic strips based on colorants of aptamer-functionalized nanogold particles weredeveloped for the detection of micropollutants aflatoxin B1 (AFB1) and chloramphenicol (CAP). The lateral flow immunochromatographic strip was based on a competitive reaction of thiolated-aptamer between micropollutants and bio-DNA probe-streptavidin as capture material immobilized at the test line. General crucial parameters that might influence the sensitivity have been systematically investigated. To test the effectiveness and applicability of the optimized conditions, two structurally unrelated micropollutants, that is, AFB1 and CAP, were chosen for detection. In the present study, lateral flow immunochromatographic strips for AFB1 and CAP analysis by combining the high selectivity and affinity of aptamers with the unique optical properties of nanogold in municipal water samples were reported for the first time. With the optimized conditions, the immunochromatographic strip showed a visual LOD of 10 ppb and a quantitative LOD of 1.05 ppb using an immunochromatographic reader for AFB1 detection and a quantitative LOD of 63.4 ppb using an immunochromatographic reader for CAP detection. Furthermore, the sensitive strip provided a good linear detection range of approximately 0-50 ppm for AFB1 detection and a wider liner detection range of approximately 0-160 ppm for CAP detection. Moreover, the immunochromatographic strip provided recovery rates for water samples of 90-110% in the AFB1 analysis and 84-108% in the CAP analysis. The results demonstrated that the immunochromatographic strip has excellent potential for wide applicability and verified that the strip methods for the optimized conditions are applicable to a variety of micropollutants. The lateral flow immunochromatographic strip could be used as a simple, rapid, and efficient screening tool for rapid on-site detection of a variety of micropollutants.
作者：Zhang, S；Zhao, S；Wang, S；Liu, JH；Dong, YY
作者单位：Beijing Univ Chem Technol
期刊名称：JOURNAL OF AOAC INTERNATIONAL
期刊影响因子：0.918
出版年份：2018
出版刊次：5
点击下载：用适体功能化的纳米金粒子着色剂研制侧向横流免疫层析试纸条检来测微量污染物，II：用黄曲霉毒素B1和氯霉素进行实验验证