中文摘要：脱氧雪腐镰刀菌烯醇（DON）是由许多谷物及食品中不同镰刀菌分泌的最重要的真菌毒素之一。它能严重损害人体和动物的免疫系统。开展本体外研究，旨在评估银纳米颗粒(Ag-NPs)对抑制从大麦、小麦和玉米粒中分离的镰刀菌分泌DON毒素的影响。研究表明，Ag-NPs可能用来替代杀菌剂，减少真菌生长并消除DON毒素。
外文摘要：Background: Deoxynivalenol (DON) is one of the most important fungal mycotoxins excretedby different Fusarium species in many types of grains and food commodities. It has high damage impact on human and animal immune systems. Objective: This in vitro study aimed to evaluate the influence of silver nanoparticles (Ag-NPs) as an inhibitor for the DON toxin excreted from some Fusarium spp., which were isolated from barely, wheat, and corn grains. Methods: Ag-NPs were estimated on Minimum Inhibitory Concentration, using levels of 5, 25, 50, 75, and 100 ppm, while the effect on DON was conducted with ELISA. Tri13 and Tri7 primers were used to evaluate the impact of Ag-NPs on the DNA of tested toxigenic Fusarium isolates. Results: Results revealed that the relative density values (Rd, %) of the isolated Fusarium from barley, wheat, and corn grains were 41.27, 26.47, and 30.76%, respectively. The predominant fungus was F. graminearum and F. culmorum in wheat and barley, respectively. The maximum inhibition diameters used for concentrations were 0.5, 2.8, 3.2, 3.3, and 3.31 mm, respectively. The impact of Ag-NPs on genomic structure was limited. Results demonstrated that Ag-NPs have the ability to reduce the linear growth of Fusarium spp. and eliminate the DON toxin to 34.44, 34.60, and 34.89% at 50, 75, and 100 ppm. Conclusions: Ag-NPs are considered nontransgenic substances, and their impact on Fusarium DNA under tested concentrations has been neglected. Ag-NPs may work as an alternative to fungicides to reduce fungal growth and eliminate DON mycotoxins.
作者：El-Naggar, MA；Alrajhi, AM；Fouda, MM；Abdelkareem, EM；Thabit, TM；Bouqellah, NA
作者单位：Agr Res Ctr
期刊名称：JOURNAL OF AOAC INTERNATIONAL
期刊影响因子：0.918
出版年份：2018
出版刊次：5
点击下载：银纳米颗粒对某些谷物产毒镰刀菌和脱氧雪腐镰刀菌烯醇分泌的影响