中文摘要：本研究合成了用两亲性N-烷基化聚（乙亚胺）包被的高荧光氨基功能化CdSe/ZnS核/壳量子点(CdSe/ZnS QDs)，并将其作为侧向横流免疫分析(LFIA)试纸的荧光探针，现场测定黄曲霉毒素B1(AFB1）。重现性和回收试验表明，该方法定量结果准确，可以用来现场快速检测谷物制品中的AFB1。
外文摘要：In this study, highly fluorescent amino-functionalized CdSe/ZnS core/shell quantum dots (CdSe/ZnS QDs) coated with amphiphilic N-alkylated poly(ethyleneimine) have been synthesized and applied as fluorescent probes in lateral flow immunoassay (LFIA) strips for on-site determination of aflatoxin B1 (AFB1). The amino-functionalized QDs were used to combine with anti-AFB1 via thiol-maleimide conjugation and they effectively enhanced the detection sensitivity. The test strip had high sensitivity for AFB1 from 0.001 ng mL−1 to 10 ng mL−1 with a low limit of detection (LOD) of 0.005 ng mL−1, which was much lower than the allowable concentration by the European Commission (EC) (2.0 μg kg−1) and FDA (20 μg kg−1). The IC50 value was calculated to be 0.025 ng mL−1. The reproducibility and recovery tests showed that the quantitative results were accurate, and it could be used for rapid on-site detection of AFB1 in cereal products.
作者：Li, JY；Mao, M；Wu, F；Li, Q；Wei, LY；Ma, L
作者单位：Tsinghua Univ
期刊名称：ANALYTICAL METHODS
期刊影响因子：1.915
出版年份：2018
出版刊次：29
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