中文摘要：本研究通过简单的水沉淀法，室温下制备了花状氧化锌(ZnO)。通过UV-可见光谱、UV-可见漫反射光谱(DRS)、荧光光谱、傅立叶变换红外光谱(FTIR) 衰减全反射(ATR)、X-射线衍射(XRD)及场发射扫描电镜(FESEM)表征了该原生纳米结构。并在体外和原位条件下，用高毒力黄曲霉菌株进一步探索了ZnO纳米结构的抗菌及抗黄曲霉毒素产生的活性。研究表明，ZnO纳米结构展示了很强的抗菌及抗黄曲霉毒素产生的活性，在农业食品体系中有潜在应用。
外文摘要：Flower-shaped zinc oxide (ZnO) nanostructures were prepared via a simple aqueous precipitation strategy at room temperature. The as-grown nanostructures were characterized by UV⁻vis spectroscopy, UV⁻vis diffuse reflectance spectroscopy (DRS), spectrofluorometry, Fourier transform infrared (FTIR) spectroscopy with attenuated total reflection (ATR), X-ray diffraction (XRD), and field emission scanning electron microscopy (FESEM). The antifungal and anti-aflatoxigenic activities of the ZnO nanostructures were further investigated using a highly toxigenic strain of Aspergillus flavus Link under in vitro and in situ conditions. The results showed that the A. flavus isolate was inhibited to various extents by different concentrations of ZnO nanostructures, but the best inhibitions occurred at 1.25, 2.5, and 5 mM in the culture media. At these concentrations, suppression of aflatoxin biosynthesis (99.7%) was also observed. Moreover, a reasonable reduction in the aflatoxin content (69%) was observed in maize grains treated with the lowest ZnO concentration that exhibited the strongest inhibitory activity in the liquid media. SEM micrographs clearly indicate multiple degenerative alterations in fungal morphology after treatment with ZnO such as damage of the tubular filaments, loss of hyphae shape, as well as hyphae rupture. These results suggest that flower-shaped ZnO nanostructures exhibit strong antifungal and anti-aflatoxigenic activity with potential applications in the agro-food system.
外文关键词：ZnO nanostructures；Aspergillus flavus；antifungal；anti-aflatoxigenic activity
作者：Hernandez-Melendez, D；Salas-Tellez, E；Zavala-Franco, A；Tellez, G；Mendez-Albores, A；Vazquez-Duran, A
作者单位：UNAM
期刊名称：MATERIALS
期刊影响因子：2.728
出版年份：2018
出版刊次：8
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