中文摘要：黄曲霉毒素是毒性最强的霉菌毒素之一，对人类健康构成了严重威胁。本文报道了一种新型液晶（LC）电池系统，可以使抗体与靶抗原黄曲霉毒素之间的免疫竞争反应显现出来。研究表明，该方法无需实验仪器，而试验结果可以用偏光显微镜通过肉眼观察。
外文摘要：Aflatoxin is one of the most toxic mycotoxins and a severe threat to human health. Here, we report a novel liquid crystal (LC) cell system that can be used to visualize the immune competition reaction between an antibody and the target antigen aflatoxin. The sensing system involves co-immobilization of the antibody and N, N-dimethyl-N-octadecyl (3-aminopropyl) trimethoxysilyl chloride (DMOAP) on the surface of a glass slide, resulting in a random orientation of the LCs in the film on these surfaces. In this system, the LC molecules are disordered, producing bright optical signals upon binding of the antibody to the surface-immobilized antigen. In the presence of aflatoxin, the interaction between the target antigen and antibody results in an immune competition reaction, which disturbs the orientation of LCs. This change in orientation can be observed by the naked eye through a crossed polarizer. The results demonstrated that this method possesses high specificity with an antigen detection limit as low as 100 pg/mL. This method does not require laboratory-based instruments, and the test results can be observed with naked eyes through a polarized microscope.
外文关键词：Aflatoxin B1；Immune competition；Liquid crystals (LCs)；4-Cyano-4 '-pentylbiphenyl (5CB)
作者：An, ZF；Jang, CH
作者单位：Gachon Univ
期刊名称：MICROCHEMICAL JOURNAL
期刊影响因子：2.893
出版年份：2018
出版刊次：11
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