中文摘要：黄曲霉毒素污染食品和粮食给经济发展和公共卫生保护带来严重问题，这就使得寻求其有效的控制方法颇受关注；本研究以香豆素为唯一碳源的培养基，分离降解黄曲霉毒素B1（AFB1）的细菌，用高效液相色谱及液相色谱-质谱联用检测菌株对AFB1的降解。研究分离到一株AFB1降解活性较强(91.5%)的细菌，鉴定为韦氏杆菌DY3108。研究表明，该菌株对食品和饲料中的AFB1解毒和生物修复具有广阔的应用前景。
外文摘要： (1) Background: Aflatoxin contamination in food and grain poses serious problems both for economic development and public health protection, thus leading to a focus on an effective approach to control it; (2) Methods: Aflatoxin B1 (AFB1) degrading bacteria were isolated using a medium containing coumarin as the sole carbon source, and the biodegradation of AFB1 by the isolate was examined by high performance liquid chromatography, and liquid chromatography mass spectrometry; (3) Results: a bacterial strain exhibiting strong AFB1 degradation activity (91.5%) was isolated and identified as Bacillus velezensis DY3108. The AFB1 degrading activity was predominantly attributed to the cell-free supernatant of strain DY3108. Besides, it was heat-stable and resistant to proteinase K treatment but sensitive to sodium dodecyl sulfate treatment. The optimal temperature for the maximal degradation of AFB1 was 80 °C. Even more notable, the supernatant showed a high level of activity over a broad pH (4.0 to 11.0) and exhibited the highest degradation (94.70%) at pH 8.0. Cytotoxicity assays indicated that the degradation products displayed significantly (p 1; (4) Conclusions: B. velezensis DY3108 might be a promising candidate for exploitation in AFB1 detoxification and bioremediation in food and feed matrices.
外文关键词：mycotoxin；aflatoxin B-1；Bacillus velezensis；biodegradation；detoxification
作者：Shu, X；Wang, YT；Zhou, Q；Li, MH；Hu, H；Ma, YH；Chen, X；Ni, J；Zhao, WW；Huang, SW；Wu, LF
作者单位：Chinese Acad Sci
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2018
出版刊次：8
点击下载：通过韦氏杆菌DY3108无细胞提取物生物降解黄曲霉毒素B1，pH稳定性高，热稳定性好