中文摘要：在撒哈拉以南非洲，急需低廉而易接受的现场检测黄曲霉毒素的方法，以实现适当的食品安全管理。本研究通过现场检测来自坎帕拉6个市场的60个玉米粉样品及72个来自家庭的样品，验证了一种电化学免疫传感器。研究表明，该现场检测新方法具有可行性，并阐明了乌干达黄曲霉毒素污染的严重性。
外文摘要：In sub-Saharan Africa, there is a high demand for affordable and accessible methods for on-site detection of aflatoxins for appropriate food safety management. In this study, we validated an electrochemical immunosensor device by the on-site detection of 60 maize flour samples from six markets and 72 samples from households in Kampala. The immunosensor was successfully validated with a linear range from 0.7 ± 0.1 to 11 ± 0.3 µg/kg and limit of detection (LOD) of 0.7 µg/kg. The maize flour samples from the markets had a mean total aflatoxin concentration of 7.6 ± 2.3 µg/kg with approximately 20% of the samples higher than 10 µg/kg, which is the maximum acceptable level in East Africa. Further down the distribution chain, at the household level, approximately 45% of the total number contained total aflatoxin levels higher than the acceptable limit. The on-site detection method correlated well with the established laboratory-based HPLC and ELISA-detection methods for aflatoxin B1 with the correlation coefficients of 0.94 and 0.98, respectively. This study shows the feasibility of a novel on-site detection method and articulates the severity of aflatoxin contamination in Uganda.
外文关键词：aflatoxins；maize；households；markets；immunosensor；HPLC；ELISA
作者：Wacoo, AP；Wendiro, D；Nanyonga, S；Hawumba, JF；Sybesma, W；Kort, R
作者单位：Vrije Univ Amsterdam
期刊名称：TOXINS
期刊影响因子：3.571
出版年份：2018
出版刊次：8
点击下载：乌干达坎帕拉市场和挑选家庭的玉米粉黄曲霉毒素现场检测新方法的可行性研究