中文摘要：本研究基于单克隆抗体，研发了超灵敏免疫分析法，包括间接竞争酶联免疫分析(ic-ELISA)及侧向横流免疫层析(ICA)，用来检测食品及饲料样品中的脱氧雪腐镰刀菌烯醇。研究表明，这些方法灵敏、简单而安全，为市场上大量样品的筛选提供了辅助分析工具。
外文摘要：Ultrasensitive immunoassays, including an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic assay (ICA), were developed based on a monoclonal antibody for the analysis of deoxynivalenol in food and feed samples. With 0.01 M PBS, 20% ethanol–PBS, and 60% ethanol–PBS extraction, which are environmentally safe, the 50% inhibitory concentration (IC50) and limit of detection (LOD) values were 1.83–4.68 μg/kg and 0.241–0.664 μg/kg, respectively, with recovery rates of 87.7%–137% and coefficient variation values of 3.99–9.88% (intra-assay) and 4.17–9.81% (inter-assay) for the ic-ELISA relative to the results obtained by liquid chromatography–tandem mass spectrometry (LC–MS). For the ICA strip, the visual LODs were 10–150 μg/kg, the cut-off values were 50–750 μg/kg, and the calculated LODs were 1.97–46.8 μg/kg, with different sample extraction solutions, and the recovery rates were 66.7%–127%. These methods are sensitive, simple and safe, providing an auxiliary analytical tool for screening the massive samples in markets.
外文关键词：Deoxynivalenol；Monoclonal antibody；Enzyme-linked immunosorbent assay；Lateral-flow immunochromatographic assay；Cereal；Feed
作者：Kong, DZ；Wu, XL；Li, Y；Liu, LQ；Song, SS；Zheng, QK；Kuang, H；Xu, CL
作者单位：Jiangnan Univ
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2019
出版刊次：1
点击下载：用基于单克隆抗体的超灵敏环境友好的免疫分析法检测谷物和饲料样品中的脱氧雪腐镰刀菌烯醇