中文摘要：脱氧雪腐镰刀菌烯醇（DON）是主要由镰刀菌产生的霉菌毒素，最常出现在谷物及谷物制品中。麦麸是麦粒的外层，化学危害，包括霉菌毒素的风险很高。亟须麦麸DON的快速检测方法。本研究用基于金属氧化物半导体传感器的电子鼻(e-鼻)，开发了一种快速筛查法，区分DON污染程度不同的麦麸样品。结果表明，该e-鼻法可能是高通量筛查DON污染麦麸样品的有用工具，它以DON污染水平接近EU最大限量进行可接受/不可接受分类，减少确证性分析的样品数量。
外文摘要：BACKGROUND: Deoxynivalenol (DON) is the mycotoxin, mainly produced by Fusarium species, most frequently occurring in cereals and cereal-based products. Wheat bran is the outer layers of the kernel with a high risk of chemical hazards, including mycotoxins. Rapid methods for DON detection in wheat bran are highly demanded. RESULTS: A rapid screening method using an electronic nose (e-nose) based on metal oxide semiconductor sensors has been developed to distinguish wheat bran samples with different levels of DON contamination. A total of 470 naturally contaminated wheat bran samples was analysed by e-nose analysis. Wheat bran samples were divided in two contamination classes: class A ([DON] ≤ 400 μg kg-1 , 225 samples) and class B ([DON] > 400 μg kg-1 , 245 samples). Discriminant Function Analysis (DFA), classified wheat bran samples with good mean recognition abilities in both calibration (92%) and validation (89%). In addition, a pattern of seventeen volatile compounds of wheat bran samples that were associated (positively or negatively) with DON content was characterized by HS-SPME/GC-MS. CONCLUSIONS: These results indicate that the developed e-nose method could be a useful tool for high throughput screening of DON-contaminated wheat bran samples for their classification as acceptable/rejectable at contamination levels close to the EU maximum limit for DON, then reducing the number of samples to be analysed with a confirmatory method.
外文关键词：electronic nose；metal oxide sensors；deoxynivalenol；wheat bran；discriminant function analysis；volatile compounds
作者：Lippolis, V；Cervellieri, S；Damascelli, A；Pascale, M；Di Gioia, A；Longobardi, F；De Girolamo, A
作者单位：CNR Natl Res Council Italy
期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
期刊影响因子：2.076
出版年份：2018
出版刊次：13
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