中文摘要：本研究提出了一种无标记光学传感器，用来同时快速测定啤酒样品中的三种霉菌毒素，黄曲霉毒素B1(AFB1)、伏马菌素B1 (FB1)及脱氧雪腐镰刀菌烯醇(DON)。用该传感器分析了不同类型及产地的啤酒，与成熟的实验室测定方法结果进行比较，进一步证实了该传感器的精度。
外文摘要：A label-free optical biosensor for the fast simultaneous determination of three mycotoxins, aflatoxin B1 (AFB1), fumonisin B1 (FB1) and deoxynivalenol (DON), in beer samples is presented. The biosensor is based on an array of ten Mach-Zehnder interferometers (MZIs) monolithically integrated along with their respective broad-band silicon light sources onto a single chip. Multi-analyte determination is accomplished by functionalizing the sensing arms of individual MZIs with mycotoxin-protein conjugates. Assay is performed by pumping over the chip mixtures of calibrators or samples with a mixture of specific monoclonal antibodies, followed by reaction with a secondary anti-mouse IgG antibody. Reactions are monitored in real-time by continuously recording the MZI output spectra, which are then subjected to Discrete Fourier Transform to convert spectrum shifts to phase shifts. The detection limits achieved for AFB1, FB1 and DON were 0.8, 5.6 and 24 ng/ml, respectively, while the assay duration was 12 min. Recovery values ranging from 85 to 115% were determined in beer samples spiked with known concentrations of the three mycotoxins. In addition, beers of different types and origin were analysed with the biosensor developed and the results were compared with those provided by established laboratory methods, further supporting the accuracy of the proposed device.
外文关键词：Aflatoxin B-1；Fumonisin B-1；Deoxynivalenol；Monolithically integrated Mach-Zehnder interferometers；Beer
作者：Pagkali, V；Petrou, PS；Makarona, E；Peters, J；Haasnoot, W；Jobst, G；Moser, I；Gajos, K；Budkowski, A；Economou, A；Misiakos, K；Raptis, I；Kakabakos, SE
作者单位：NCSR Demokritos
期刊名称：JOURNAL OF HAZARDOUS MATERIALS
期刊影响因子：4.836
出版年份：2018
出版刊次：10
点击下载：用无标记单片集成光电生物传感器同时检测啤酒样品中的黄曲霉毒素B1、伏马菌素B1及脱氧雪腐镰刀菌烯醇