中文摘要：本研究研发了一种基于适体的芯片毛细管电泳结合激光诱导的荧光检测法，用来快速测定黄曲霉毒素B1（AFB1）和赭曲霉毒素A（OTA）。研究表明，该方法快速、简便，能同时从食品中特异性检测出痕量AFB1及OTA。
外文摘要：An aptamer-based microchip capillary electrophoresis coupled with laser induced fluorescence (MCE-LIF) detection method for fast determination of Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) was developed. Aptamers that are specific to these two mycotoxins were first hybridized with their aptamer complementary oligonucleotides. The double strand DNA that comes in contact with mycotoxin-containing environment would be unwound into separate aptamer-mycotoxin complex and aptamer complementary single strand. Different types of oligonucleotides can be separated in MCE and detected under the aid of fluorescent dye SYBR gold in LIF detection unit. Under the optimal conditions, on-chip aptamer-mycotoxin conjugates analysis was achieved within 3 min with extremely low LODs (0.026 ng/mL for AFB1 and 0.021 ng/mL for OTA). Specificity study indicated that other major mycotoxins would not cross-react with these two aptamers, demonstrating the good selectivity of the proposed method. Quantification of trace AFB1 and OTA in real food samples was carried out and satisfactory recoveries were obtained. It is demonstrated that this method is fast, facile and specific for Simultaneous determination of trace AFB1 and OTA from foodstuffs.
外文关键词：Microchip capillary electrophoresis；Laser induced fluorescence；Aptamer；Aflatoxin B-1；Ochratoxin A
作者：Xiao, MW；Bai, XL；Liu, YM；Yang, L；Liao, X
作者单位：Chinese Acad Sci
期刊名称：JOURNAL OF CHROMATOGRAPHY A
期刊影响因子：3.926
出版年份：2018
出版刊次：9
点击下载：通过基于适体的芯片毛细管电泳，同时检测食品样品中的痕量黄曲霉毒素B1和赭曲霉毒素A