中文摘要：本研究首次制备了抗玉米赤霉烯酮（ZEN）的单克隆抗体(mAb) (2B10)。该mAb具有种类特异性，不与其它霉菌毒素发生交叉反应。提出了一种基于新型固体支撑材料，聚二甲基硅氧烷(iPDMS)的竞争性免疫分析法，用来定量/半定量检测谷物样品中的ZEN。值得注意的是，与ELISA比较，该芯片法快速、简单，且无需阻断。此外，由于其零背景的优点，适当增加包被抗原及辣根过氧化物酶(HRP)标记的二抗浓度，能提高膜灵敏度并减少样品的基质效应。
外文摘要：A monoclonal antibody (mAb) (2B10) against zearalenone (ZEN) was prepared for the first time in this study. The mAb was class specific and showed no cross reactivity to other groups of mycotoxins. A competitive microarray assay based on a novel solid supporting material, an integrated poly(dimethylsiloxane) (iPDMS), is proposed for qualitative and/or semiquantitative determination of ZEN in cereal samples. The limit of detection (LOD) and limit of quantification (LOQ) of the iPDMS based method were 0.53 g kg(-1) and 1.02 g kg(-1) for ZEN, respectively, which was lower than the EU maximum limit (ML). Furthermore, recoveries ranged from 93% to 114% and the coefficients of variation were below 15%. The developed method was successfully applied to analyse 29 cereal samples and the results showed that three corn flour samples were ZEN positive, and two corn flour samples were determined to be suspicious, which was in a good agreement with the results obtained using LC-MS/MS. It is worth noting that, compared with ELISA, the developed microarray assay is rapid, simple and does not require a blocking step. In addition, owing to its advantage of zero background, appropriately increasing the concentration of coating antigen and horseradish peroxidase (HRP)-labeled secondary antibody can enhance the sensitivity of the membrane and reduce the matrix effects of the sample.
作者：Li, L；Ren, S；Shao, MY；Saeger, S；Song, SQ；Yan, LP
作者单位：Nanjing Agr Univ
期刊名称：ANALYTICAL METHODS
期刊影响因子：1.915
出版年份：2018
出版刊次：33
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