中文摘要：以多孔g-C3N4纳米片(pg-CNNSs)作荧光探针，AFB1-牛血清白蛋白偶联物修饰的Fe3O4磁性纳米粒子(MNPs)作免疫传感探针，首次构建了一种简便灵敏的黄曲霉毒素B1(AFB1)检测法。研究表明，最佳条件下该方法对AFB1的检测限低至0.002 ng/mL。此外，通过检测掺入次品级的玉米样品，印证了该方法。
外文摘要：With the porous g-C3N4 nanosheets (pg-CNNSs) as fluorescence probes and AFB1–bovine serum albumin conjugate modified Fe3O4 magnetic nanoparticles (MNPs) as immunosensing probes, a simple sensitive aflatoxin B1 (AFB1) detection method was first constructed. Based on the simple preparation method, the excellent biocompatibility, strong fluorescence emission and proper emission wavelength, pg-CNNSs were first functionalized by AFB1 antibody as fluorescence probes, which can bind with MNPs through antigen-antibody immunoreaction to form the pg-CNNSs-MNPs complexes. After magnetic separation, the fluorescence intensity of the complexes was tested. The target analyte (AFB1) can interfere the above immunoreaction by direct competition with immunosensing probes, and lead to the signal decreased. The detection limit for AFB1 was as low as 0.002 ng mL−1 under optimal conditions. Additionally, the applicability of this method was also confirmed in adulterated maize samples.
外文关键词：Fluorescence immunoassay；Aflatoxin B1；Porous g-C3N4 nanosheets；Magnetic nanoparticles
作者：Xie, HZ；Dong, J；Duan, JL；Hou, JY；Ai, SY；Li, XY
作者单位：Shandong Agr Univ
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL
期刊影响因子：4.758
出版年份：2019
出版刊次：1
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