中文摘要：本研究提出了一种小型化固相萃取法，用来提取牛奶及红枣样品中的黄曲霉毒素M1和B1。并通过超高效液相色谱-四极杆飞行时间串联质谱法，以正离子模式对目标分析物进行识别。结果表明，该方法具有简单、廉价、快速而精确等显著优势，因而被认为是适用于痕量黄曲霉毒素分析的方法。
外文摘要：A miniaturized solid-phase method was proposed for the extraction of aflatoxin M1 and B1 from milk and jujube samples. Target analytes were identified by ultra high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry in positive mode. The main factors that affected the extraction ability and sensitivity of the analytical method were evaluated in detail. In the optimal conditions, 30 mg of silica particles functionalized with octadecyltrimethoxysilane and methanol were used as sorbent and desorption solvent in miniaturized solid-phase extraction, respectively. The calibration curves for aflatoxin B1 and M1 showed good linearity (r2 > 0.9921) within linear ranges. The limit of detection (S/N = 3) were determined to be 0.049 and 0.023 μg/kg for aflatoxin M1 and B1, respectively. In addition, the good spiked recoveries were obtained ranging from 88.24 to 105.35%. The results demonstrated that the proposed method had significant advantages including simple, inexpensive, rapid, and accurate, which considered as a suitable method for the trace analysis of aflatoxins.
外文关键词：aflatoxins；mass spectrometry；microextraction；solid-phase extraction；ultra high performance liquid chromatography
作者：Du, LJ；Wang, SL；Huang, JP；Chu, C；Li, RR；Li, Q；Wang, QY；Hu, YH；Cao, J；Chen, YB；Peng, LQ；Yang, J
作者单位：Hangzhou Normal Univ
期刊名称：JOURNAL OF SEPARATION SCIENCE
期刊影响因子：2.741
出版年份：2018
出版刊次：10
点击下载：通过微型固相萃取-超高效液相色谱-四极飞行时间串联质谱联用测定牛奶及红枣中的黄曲霉毒素M1和B1