中文摘要：本研究研发了一种流动注射电容型免疫传感器，其检测玉米赤霉烯酮(ZEN)的灵敏度很高。研究证实，与直接微孔板型免疫传感器相比，该无标记电容型免疫传感器的灵敏度更高且更省时。
外文摘要：A highly sensitive flow-injection capacitive immunosensor was developed for detection of the mycotoxin zearalenone (ZEN). Different strategies for immobilization of an anti-ZEN antibody on the surface of a gold electrode, i.e. polytyramine or self-assembled monolayers (SAMs) of 3-mercaptopropionic acid (3-MPA) and lipoic acid (LA), were used and their performances were compared. The LA- and 3-MPA-based systems showed broad linear ranges for ZEN determination, i.e. from 0.010 nM to 10 nM and from 0.020 nM to 10 nM, respectively. Under optimal conditions, the LA-based immunosensor was capable of performing up till 13 regeneration-interaction cycles (with use of glycine HCl, pH 2.4) with a limit of detection (LOD) of 0.0060 nM, equivalent to 1.9 pg mL−1. It also demonstrated a good inter-assay precision (RSD 15%) which did not allow sensitive and precise measurements. The LA-based method was compared with a direct ELISA. These results demonstrated that the label-free developed capacitive immunosensor had a better sensitivity and shorter analysis time in comparison with the direct microwell-plate format.
外文关键词：Capacitive immunosensor；Folytyrarnine；Self-assembled monolayers；Zearalenone；Mycotoxin
作者：Foubert, A；Beloglazova, NV；Hedstrom, M；De Saeger, S
作者单位：Univ Ghent
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2019
出版刊次：1
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