中文摘要：本研究提出了一种检测食源性毒素玉米赤霉烯酮(ZEN)的基于印迹蛋白的免疫分析设计策略。研究表明，用该免疫方法分析ZEN，其灵敏性能满足检测欧盟建立的未加工谷物中ZEN最大允许水平(100 μg/ kg)的需要。
外文摘要：A strategy to design an immunoassay based on imprinted proteins to detect a foodborne toxin zearalenone (ZEN) has been proposed. Proteins were used as scaffolds for generation of binding cavities with a high specificity against ZEN. Different proteins and different bioimprinting approaches were tested. The imprinted proteins primarily prepared in a tube and then immobilized on a microwell plate, and directly prepared in the microwell plate were compared for an application in immunosorbent detection of ZEN in naturally contaminated wheat and maize samples. The assay was combined with a rapid extraction technique. Specific interactions between the imprinted proteins and the target in a μg kg−1 linear range was achieved. As a confirmatory method, liquid chromatography coupled to tandem mass spectrometry was used. The obtained results allow using the developed immunoassay for the analysis of ZEN with a sensitivity matching the maximum permitted level for ZEN in unprocessed cereals established by the European Commission (100 μg kg−1).
外文关键词：Imprinted protein；Bioimprinting；ELISA；Zearalenone；Rapid screening；Mycotoxin
作者：Pidenko, P；Zhang, HY；Lenain, P；Goryacheva, I；De Saeger, S；Beloglazova, N
作者单位：Saratov NG Chernyshevskii State Univ
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：4.712
出版年份：2018
出版刊次：12
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