中文摘要：本研究描述了一种同时测定黄曲霉毒素M1（AFM1）及赭曲霉毒素A（OTA）的基于双色荧光共振能量转移(FRET)的适体传感器。用该基于FRET的双色检测法同时检测牛奶中的AFM1及OTA，获得了良好的回收率及精准度。
外文摘要：A dual-color fluorescence resonance energy transfer (FRET) based aptasensor is described for simultaneous determination of the mycotoxins aflatoxin M1 (AFM1) and ochratoxin A (OTA). Aptamers against AFM1 and OTA were labeled with two fluorophores with different excitation wavelengths (Cy5.5; 675 nm; and Alexa 405; 401 nm), respectively. They were used as the signalling probes. A compact dual-color evanescent wave all-fiber detection system with two lasers (635 nm; red; and 405 nm; purple) was used for the simultaneous collection of two-wavelength fluorescence signals. The hybridization of labeled aptamers with complementary sequences (Q-cDNA) labeled with a dark quencher (BHQ3 or dabcyl) causes fluorescence to be strongly reduced because of the fluorescence resonance energy transfer. In the presence of AFM1 and OTA, they bind to their respective aptamer and result in the dissociation of double stranded DNA, which induce fluorescence recovery. Under the optimum conditions, AFM1 and OTA can simultaneously and selectively be determined ranged from 1 ng·L−1 to 1 mg·L−1. The detection limits of AFM1 and OTA are 21 and 330 ng·L−1, respectively (S/N = 3). The FRET-based dual-color detection scheme was applied to the simultaneous detection of AFM1 and OTA in milk with good recovery, precision, and accuracy.
外文关键词：Mycotoxin；Fluorescence；Total internal reflection fluorescence；Multi-wavelengths；Fiber optical switch；Resonance energy transfer；Aptamer；Food monitoring
作者：Song, D；Yang, R；Fang, SY；Liu, YP；Long, F
作者单位：Renmin Univ China
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2018
出版刊次：11
点击下载：用基于FRET的双色倏逝波光纤传感器同时荧光检测黄曲霉毒素M1和赭曲霉毒素A