中文摘要：本研究采用单一测试线和多色金纳米粒子（GNPS）作为信号报告，研发了多重侧向横流免疫分析法。通过同时检测小麦及其制品中的黄曲霉毒素B1（AFB1）及B型伏马菌素（FMs），证明了“彩色编码分析”方法功能的多样性。重复性检测生、熟食品中AFB1及FMs的可视化水平分别为1 ng/mL及50 ng/mL。该方法依据特定颜色代码，从标记的颜色来识别污染物。利用普通智能手机获取条带图像，并通过RGB数据分析，提供该两种霉菌毒素的半定量检测。
外文摘要：A multiplex Lateral Flow Immunoassay was developed based on the use of a single Test line and multicolour gold nanoparticles (GNPs) as signal reporters. Red and blue GNPs were linked to antibodies directed towards two different analytes and included in a typical lateral flow immunoassay configuration, in which the Test line was formed by the mixture of two antigens. As a result of the immunoreactions occurring at the Test zone, diverse combinations of red and blue GNPs labels were captured. Therefore, the Test line assumed different colours depending on which - and how much - analyte is present in the sample. The multiplexing capability of the 'colour-encoded assay' is illustrated by the simultaneous detection of aflatoxin B1 (AFB1) and type-B fumonisins (FMs) in wheat and food products that made with wheat. Reproducible detection of AFB1 and FMs contamination in raw and processed food was achieved with visual cut-off levels at 1 ng mL-1 and 50 ng mL-1, respectively. The contaminant was identified based on the colour of the label according with a specific colour code. Furthermore, strips images were acquired by means of a common smartphone and analysed through RGB data analysis providing semi-quantitative detection of the two mycotoxins.
外文关键词：Immunochromatographic strip test；Blue gold nanoparticles；Multiplex detection；RGB data evaluation；Smartphone；Mycotoxins
作者：Di Nardo, F；Alladio, E；Baggiani, C；Cavalera, S；Giovannoli, C；Spano,G；Anfossi, L
作者单位：Univ Turin
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2019
出版刊次：1
点击下载：用彩色编码侧向横流免疫分析法在单一测试线上同时检测黄曲霉毒素B1及B型伏马菌素