中文摘要：本研究首次提出了一种使用生物印迹蛋白，在单一位点同时定量检测两种分析物的灵敏工具。研究发现，用它同时检测小麦和玉米中的玉米赤霉烯酮及脱氧雪腐镰刀菌烯醇，检测限分别为100和700/µg kg。并用液相色谱串联质谱(LC-MS/MS)法验证了该结果。
外文摘要：A sensitive tool for simultaneous quantitative determination of two analytes in a single spot with the use of a bioimprinted protein is presented for the first time. BSA is chosen as a scaffold for generation of binding sites specific towards two compounds. A multiplex immunosorbent assay for screening of two cereal-born mycotoxins, deoxynivalenol and zearalenone, in wheat and maize is realized with the use of fluorescent silica coated quantum dots as labels. Water-soluble fluorescent nanostructures consist of core/shell Cd-QDs enrobed in silica shells to ensure their solubility. The mycotoxins are simultaneously detected by scanning the assay outcome at two different wavelengths, since two QD@SiO2 labelled conjugates fluorescent in different parts of the spectrum. The assay is combined with a rapid extraction technique. The limits of detection for the simultaneous determination were 100 and 700 µg kg-1 in both matrices for zearalenone and deoxynivalenol, respectively. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to confirm the obtained results.
外文关键词：Bioimprinting；Quantum dots；Silica coated quantum dot；Immunoassay；Zearalenone；Deoxynivalenol
作者：Beloglazova, N；Lenain, P；Tessier, M；Goryacheva, I；Hens, Z；De Saeger, S
作者单位：Univ Ghent
期刊名称：TALANTA
期刊影响因子：4.035
出版年份：2019
出版刊次：1
点击下载：检测脱氧雪腐镰刀菌烯醇和玉米赤霉烯酮的多重发光生物印迹技术