中文摘要：本文介绍了一种基于适体的玉米赤霉烯酮（ZEN）比色测定法。研究表明，玉米赤霉烯酮的浓度在10-250ng/mL范围内，吸光度（630nm）呈线性增加，检测限为10ng/mL。通过研究潜在的干扰效应，证实了该方法具有特异性。ZEN加标玉米及玉米油的回收率从92到110%，相对标准偏差在2.4 与6.4%之间。测定结果与ELISA法高度一致。
外文摘要：An aptamer based colorimetric assay is described for the determination of zearalenone (ZEN). It is based on the inhibition of the peroxidase-mimicking activity of gold nanoparticles (AuNPs) by the ZEN aptamer. However, in the presence of ZEN, the aptamer is bound by ZEN and can no longer inhibit the peroxidase-mimicking activity of AuNPs. The color change of solution is related to ZEN concentration and observed with bare eyes. Under optimal conditions, the absorbance (at 630nm) increases linearly in the ZEN concentration range of 10-250ngmL(-1), and the limit of detection is 10ngmL(-1). The specificity of the assay was verified by studying the effect of potential interferents. The recoveries from ZEN spiked corn and corn oil range from 92 to 110%, and the relative standard deviations are between 2.4 and 6.4%. The results are in good agreement with those obtained by an ELISA.
外文关键词：Zearalenone；Colorimetric assay；Mimetic enzyme；Enzyme mimicking nanomaterial；Aptasensor；Gold nanoparticles；Rapid detection；Mycotoxin analysis
作者：Sun, SM；Zhao, R；Feng, SM; Xie, YL
作者单位：Henan Univ Technol
期刊名称：MICROCHIMICA ACTA
期刊影响因子：4.831
出版年份：2018
出版刊次：12
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