中文摘要：伏马菌素最初发现于轮枝镰刀菌中，是一种在玉米中的与疾病和无症状感染相关的真菌。之后发现其他真菌类群也会产生伏马菌素。从土壤中分离出昆虫病原子囊菌纲柱孢弯颈霉，也可以作为草叶的内生菌。这项工作的目的是确定伏马菌素（FB）霉菌毒素和免疫抑制化合物环孢菌素A（CyA）在几种柱孢弯颈霉菌株中的体外生产，并调查真菌病毒感染和温度对伏马菌素生产的影响。一些真菌菌株的FB浓度同与食物和饲料中毒有关的真菌中的FB浓度相似。
外文摘要：Fumonisins were first discovered in Fusarium verticillioides, a fungus associated to disease and asymptomatic infections in maize. Afterwards, other fungal taxa have been found to produce fumonisins. The entomopathogenic ascomycete Tolypocladium cylindrosporum has been isolated from soil and also as an endophyte from leaves of grasses. The objectives of this work were to determine the in vitro production of fumonisin B (FB) mycotoxins and the immunosuppressive compound cyclosporine A (CyA) in several strains of T. cylindrosporum, and to examine the effect of fungal virus infection and temperature in FB production. FB1 was detected in 30% of the strains, ranging from 0.16 to 5.52 mu g cm(-2) in solid media, and FB2 was detected in 78% of the strains, ranging from 0.764 to 40.92 mu g cm(-2). CyA was not detected in any strain. The mean FB2 concentration of the endophytic strain Tc37W was three times greater (p < 0.05) than that of any other strain. Up to 34% more of FB2 was detected in strains infected by the virus TcV3 than in the corresponding virus-free versions. The effect of temperature on FB2 content was interactively significantly dependent on fungal strain and growth medium; in the YES medium, the FB2 of virus-infected strains Tc37-1V and Tc37W increased by 67 and 16%, respectively, at 26 degrees C as compared to 20 degrees C. The FB concentration in some fungal strains was similar to that in fungi associated to food and feed intoxications.
外文关键词：Cyclosporine A；Endophyte; Grasses；Mycovirus；Temperature effect
作者：Zabalgogeazcoa, Inigo；Alvarez, Amador；Herrero, Noemi等
作者单位：Consejo Superior de Investigaciones Cientificas
期刊名称：MYCOTOXIN RESEARCH
期刊影响因子：2.854
出版年份：2018
出版刊次：1
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