中文摘要：本研究研发了一种简便高效的方法，用来测定油样品中的黄曲霉毒素B1（AFB1）。用低温净化(LTC)-免疫磁固相萃取(IMSPE)净化样品。用该方法分析了五种实际的不同植物油样品，结果表明该方法非常灵敏而准确。
外文摘要：In the present study, a convenient and highly efficient method was developed to quantify aflatoxin B1 (AFB1) in oil samples. Low temperature clean-up (LTC) followed by immuno magnetic solid phase extraction (IMSPE) was used to clean up oil samples. LTC assisted in freezing out the interference from the oil matrix while IMSPE further helped to preconcentrate the targeted analyte. For IMSPE, we synthesised and characterised anti-AFB1 monoclonal antibody-functionalised magnetic nanoparticles. Oil sample extraction was then carried out using LTC-IMSPE. The proposed method showed satisfactory efficiency and reproducibility with recovery rates being within the range of 79.6–117.9%, with a relative standard deviation below 11.48%. The sensitivity of the method was satisfactory with the limits of detection and quantification being as low as 0.0048 and 0.0126 ng·g−1, respectively. Real sample analysis was carried out for five kinds of different vegetable oils. The results suggest that the method developed is very sensitive and accurate.
外文关键词：Vegetable oil；Food hazard；Aflatoxin；Low temperature cleanup；Nanoparticle；Immuno magnetic solid phase extraction；Fluorometric analysis；Rapid detection
作者：Yu, X；Li, ZM；Zhao, MZ；Lau, SCS；Tan, HR；Teh, WJ；Yang, HS；Zheng, C；Zhang, YQ
作者单位：Natl Univ Singapore
期刊名称：FOOD CHEMISTRY
期刊影响因子：4.052
出版年份：2019
出版刊次：3
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