中文摘要：本研究研发了一种基于生物条形码滚圆放大(RCA)的方法，用来检测T-2毒素。研究表明，该方法在超灵敏检测复杂基质中的各种小分子物质方面，具有潜在的应用前景。
外文摘要：A novel and highly sensitive method based on bio-barcode with rolling circle amplification (RCA) was developed for the detection of T-2 toxin. Gold nanoparticles (AuNPs) were modified with anti-T-2 monoclonal antibody and single-stranded thiol-oligonucleotides (SH-ssDNAs) and magnetic microparticles (MMPs) coated with T-2 antigen. The T-2 toxin competes with the antigen on MMPs for the anti-T-2 antibody on AuNPs. Then, the isolating complex system was separated by a magnetic field, and the DNA of the probes was released after washing in dithiothreitol solution. The barcode DNA via RCA and products were stained by SYBR Green I and then detected by fluorescence spectrophotometry. The optimized method was performed on oats, millet, flour, and other substances. This method exhibits a low limit of detection (0.26 pg mL−1) and linear range of 0.002–200 ng mL−1. Moreover, the approach offers good recovery and relative standard deviations ranging from 88.65%–10.04% and 0.6%–13.1%, respectively. In conclusion, this method exhibits potential for use as an ultrasensitive assay for the detection of a variety of small molecules in complex matrices.
外文关键词：Bio-barcode；T-2 toxin；Rolling circle amplification；Gold nanoparticles；Magnetic nanoparticles；Fluorescence
作者：Zhang, M；Huo, BY；Yuan, S；Ning, BA；Bai, JL；Peng, Y；Liu, BL；Gao, ZX
作者单位：Univ Shanghai Sci & Technol
期刊名称：ANALYTICA CHIMICA ACTA
期刊影响因子：4.712
出版年份：2018
出版刊次：12
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