中文摘要：本研究使用毛细管阵列，研发了一种简便的适体荧光法，用来检测黄曲霉毒素B1（AFB1）。该检测方法在食品安全分析和环境监测中具有广阔的应用前景。
外文摘要：We developed a simple aptamer fluorescence assay for aflatoxin B1 (AFB1) detection by using an array of capillaries. The 34-nt aptamer having a single fluorescein (FAM) label on the 24th T nucleotide generated a remarkable fluorescence increase upon AFB1 binding. The use of fluorescence scanning capillary array allowed for the analysis of multiple samples with low sample consumption, showing advantages of simplicity, rapidity and high throughput analysis. The detection limit of AFB1 reached 0.5 nM. This assay has great potential for analysis in food safety and environmental monitoring.
作者：Sun, LL；Zhao, Q
作者单位：Chinese Acad Sci
期刊名称：ANALYST
期刊影响因子：4.033
出版年份：2018
出版刊次：19
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