中文摘要：本研究建立了一个模型来阐明硬粒小麦中玉米赤霉烯酮（ZEN）的摄取，易位和代谢转归。用ZEN处理后，用LC-HRMS对根和芽进行了形态分析。对植物体内ZEN的生物转化和该模型的生物技术评价进行了全面描述。该研究强调了在没有镰刀菌持续感染的植物中出现ZEN的可能性。表明微繁殖可能是研究霉菌毒素和小麦之间相互作用的一种工具。
外文摘要：A model was set up to elucidate the uptake, translocation, and metabolic fate of zearalenone (ZEN) in durum wheat. After treatment with ZEN, roots and shoots were profiled with LC-HRMS. A comprehensive description of in planta ZEN biotransformation and a biotechnological evaluation of the model were obtained. Up to 200 mu g ZEN were removed by each plantlet after 14 days. Most ZEN and its masked forms were retained in roots, while minimal amounts were detected in leaves. Sixty-two chromatographic peaks were obtained, resulting in 7 putative phase I and 18 putative phase II metabolites. ZEN16G1c and ZEN14G1c were most abundant in roots, sulfo-conjugates and zearalenol derivatives were unable to gain systemic distribution, while distinct isomers of malonyl conjugates were found in leaves and roots. This study underlines the potential ZEN occurrence in plants without an ongoing Fusarium infection. Micropropagation may represent a tool to investigate the interplay between mycotoxins and wheat
外文关键词：risk assessment zearalenone；biotransformation；masked mycotoxins；biofactories
作者：Rolli, Enrico；Righetti, Laura；Galaverna, Gianni等.
作者单位：University of Parma
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
期刊影响因子：3.412
出版年份：2018
出版刊次：6
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