中文摘要：单端孢霉烯族毒素是由多种镰刀菌菌种产生的一组结构相关的倍半萜类代谢物，其经常污染谷物并威胁人类和动物健康。该霉菌毒素组的基本结构是一种特有的12,13-环氧基团，是毒性的必需官能团。在本研究中，利用单端孢霉烯族毒素-脱氧雪腐镰刀菌烯醇（DON）作为代表性基质，我们从鸡肠中鉴定出一种新型的单端孢霉烯深度氧化细菌-伊格尔兹氏菌 DII-9（DII-9）。 DII-9可以在20-45摄氏度范围内和5-10pH值范围内产生和转化DON，这表明未来有可能开发出有前景的饲料添加剂。底物特异性分析表明DII-9能够促进DON，HT-2，T-2三醇和T-2四醇的深度氧化。为了探索单端孢霉烯脱环氧化的分子机制，对DII-9的完整基因组进行了测序和表征。总之，本研究鉴定和表征了一种用于单端孢霉烯族毒素的新型解毒细菌。
外文摘要：Trichothecene mycotoxins are a group of structurally related sesquiterpenoid metabolites produced by multiple Fusarium species that often contaminate cereals and threaten human and animal health. The basic structure of this mycotoxin group is a characteristic 12, 13-epoxide group, which is considered an essential functional group for toxicity. In this study, using trichothecene mycotoxin deoxynivalenol (DON) as a representative substrate, we identified a novel trichothecene deepoxidation bacterium, Eggerthella sp. DII-9 (DII-9), from chicken intestines. DII-9 can grow and transform DON over abroad range of temperatures (20-45 degrees C) and pH values (5-10), suggesting the possibility of developing promising future applications as feed additives. Substrate specificity analysis showed that DII-9 is capable of promoting the deepoxidation of DON, HT-2, T-2 triol and T-2 tetraol. To explore the molecular mechanisms of the de-epoxidation of trichothecenes, the complete genome of DII-9 was sequenced and characterized. Altogether, a novel detoxification bacterium for trichothecene mycotoxins was identified and characterized.
外文关键词：Trichothecenes；De-epoxidation；Eggerthella sp Dll-9；Genome
作者：Gao, Xiaojuan；Mu, Peiqiang；Wen, Jikai等.
作者单位：South China Agricultural University
期刊名称：FOOD AND CHEMICAL TOXICOLOGY
期刊影响因子：3.977
出版年份：2018
出版刊次：-
点击下载：新型细菌伊格尔兹氏菌 DII-9对单端孢霉烯族毒素的解毒作用