中文摘要：轮枝镰孢菌是一种主要的玉米病菌和伏马菌素的生产者，其与人和动物的毒性效应有关。传统的基于形态特征检测真菌污染的方法耗时、灵敏度低且特异性低。因此，本研究旨在开发一种基于FUM21基因的PCR-ELISA方法，用于轮枝镰孢菌的检测。试验中，利用传统PCR技术和PCR-ELISA技术分析轮枝镰孢菌、镰刀菌、曲霉菌和青霉菌菌株的DNA，以测定其特异性。结果表明，本研究建立的PCR-ELISA方法可用于玉米样品中轮枝镰孢菌的检测。
外文摘要：Fusarium verticillioides is a primary corn pathogen and fumonisin producer which is associated with toxic effects in humans and animals. The traditional methods for detection of fungal contamination based on morphological characteristics are time-consuming and show low sensitivity and specificity. Therefore, the objective of this study was to develop a PCR-ELISA based on the FUM21 gene for F. verticillioides detection. The DNA of the F. verticillioides, Fusarium sp., Aspergillus sp. and Penicillium sp. isolates was analyzed by conventional PCR and PCR-ELISA to determine the specificity. The PCR-ELISA was specific to F. verticillioides isolates, showed a 2.5 pg detection limit and was 100-fold more sensitive than conventional PCR. In corn samples inoculated with F. verticillioides conidia, the detection limit of the PCR-ELISA was 1 x 10(4) conidia/g and was also 100-fold more sensitive than conventional PCR. Naturally contaminated corn samples were analyzed by PCR-ELISA based on the FUM21 gene and PCR-ELISA absorbance values correlated positively (p < 0.05) with Fusarium sp. counts (CFU/g). These results suggest that the PCR-ELISA developed in this study can be useful for F. verticillioides detection in corn samples.
外文关键词：Toxigenic fungi；Food-borne fungi；PCR-ELISA；Fumonisin；Corn；Mycotoxin
作者：Omori, Aline Myuki；Sataque Ono, Elisabete Yurie；Bordini, Jaqueline Gozzi 等.
作者单位：Universidade Estadual de Londrina
期刊名称：FOOD MICROBIOLOGY
期刊影响因子：4.09
出版年份：2018
出版刊次：-
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